Linked Life Data

106731

Source:http://linkedlifedata.com/resource/pubmed/id/106731

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1979-5-23
pubmed:abstractText
The maximum bactericidal capacity of neutrophils from a patient with the Chediak-Higashi syndrome (CHS) was measured by a quantitative assay in which the neutrophils were challenged with increasing multiples of Staphylococcus aureus, 502A. At various bacterial challenges from 0.5 to 65 bacteria per neutrophil, the CHS cells killed normal numbers of bacteria in 60 minutes. However, at higher ratios with a mean of 118 bacteria per neutrophil, the percentage of bacteria killed in 60 minutes by CHS neutrophils (8.1 +/- 2.3%) was significantly less (P < 0.001) than that killed by normal neutrophils (44 +/- 2.3%). The CHS neutrophils lagged in their ability to kill low challenges of bacteria (0.5 or 1.2 bacteria per neutrophil) only at 20 minutes. A hydrogen-peroxide-producing strain of Streptococcus faecalis was killed normally by the CHS neutrophils at 60 minutes, with all ratios of challenge up to 114 to 1. Electron microscopic examination of 60-minute specimens from high ratios of challenge that were stained for myeloperoxidase activity revealed a failure of many bacteria-laden phagosomes to display this marker of degranulation. The results of this study indicate that the maximal bactericidal capacity of CHS neutrophils is saturated by a significantly lower challenge of bacteria than is required to saturate normal cells. This appears to be the result of sequestration of a significant portion of these cells' bactericidal resources in the giant granules that do not participate appreciably in degranulation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-1092386, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-1113502, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-185307, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-408453, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-4550589, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-4942966, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-5007151, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-5009975, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-5104888, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-5339467, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-5913047, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-6023060, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-956687, http://linkedlifedata.com/resource/pubmed/commentcorrection/106731-978041
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
539-47
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
The Chediak-Higashi syndrome: quantitation of a deficiency in maximal bactericidal capacity.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.