10669759

pubmed:Citation

5

The C-to-U editing of apolipoprotein B (apo-B) mRNA is catalyzed by a multiprotein complex that recognizes an 11-nucleotide mooring sequence downstream of the editing site. The catalytic subunit of the editing enzyme, apobec-1, has cytidine deaminase activity but requires additional unidentified proteins to edit apo-B mRNA. We purified a 65-kDa protein that functionally complements apobec-1 and obtained peptide sequence information which was used in molecular cloning experiments. The apobec-1 complementation factor (ACF) cDNA encodes a novel 64.3-kDa protein that contains three nonidentical RNA recognition motifs. ACF and apobec-1 comprise the minimal protein requirements for apo-B mRNA editing in vitro. By UV cross-linking and immunoprecipitation, we show that ACF binds to apo-B mRNA in vitro and in vivo. Cross-linking of ACF is not competed by RNAs with mutations in the mooring sequence. Coimmunoprecipitation experiments identified an ACF-apobec-1 complex in transfected cells. Immunodepletion of ACF from rat liver extracts abolished editing activity. The immunoprecipitated complexes contained a functional holoenzyme. Our results support a model of the editing enzyme in which ACF binds to the mooring sequence in apo-B mRNA and docks apobec-1 to deaminate its target cytidine. The fact that ACF is widely expressed in human tissues that lack apobec-1 and apo-B mRNA suggests that ACF may be involved in other RNA editing or RNA processing events.

http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-1371931, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-1400437, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-1464582, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-1649450, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-1675426, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-1885564, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-1939106, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-1996349, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-2216773, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-2254300, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-2758465, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-3621347, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-3659919, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-7510636, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-7736571, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-7782342, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-7862132, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-7926751, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8036511, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8051066, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8063816, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8078915, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8119937, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8208612, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8246950, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8407891, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8409768, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8419928, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8463271, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8511591, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8576187, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8577721, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8779443, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8833435, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8909139, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8910449, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-8999813, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-9084497, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-9175473, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-9326486, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-9346911, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-9466941, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-9671452, http://linkedlifedata.com/resource/pubmed/commentcorrection/10669759-9792439

http://linkedlifedata.com/resource/pubmed/journal/8109087

http://linkedlifedata.com/resource/pubmed/chemical/AICDA (activation-induced cytidine..., http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins B, http://linkedlifedata.com/resource/pubmed/chemical/Cytidine Deaminase, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/apolipoprotein B mRNA editing enzyme

Mar

pubmed-author:DriscollD MDM, pubmed-author:KinterM TMT, pubmed-author:MehtaAA, pubmed-author:ShermanN ENE

20

Y

2009-11-18

2000

Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.