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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-2-29
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pubmed:abstractText |
Mammalian cells produce both N-arachidonoylethanolamine (20:4n-6 NAE, anandamide) and 2-arachidonoylglycerol (2-AG), lipid signaling molecules that activate cannabinoid receptors. Because both agonists occur in the presence of receptor-inactive congeners, we have developed a sensitive method for the simultaneous assay of N-acylethanolamines (NAEs) and 2-monoacylglycerols (2-MAG). These lipid classes are isolated from total lipids by solid phase extraction and converted to tert-butyldimethylsilyl (tBDMS) derivatives in the presence of deuterated analogs. The tBDMS derivatives are analyzed by gas chromatography/mass spectrometry using selected ion monitoring programs specific for NAE and 2-MAG. Individual NAEs and 2-MAGs can be quantified in the nanogram and subnanogram range. The NAE and 2-MAG compositions of rat organs and cultured JB6 cells are reported.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0009-3084
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
185-91
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10669310-Animals,
pubmed-meshheading:10669310-Arachidonic Acids,
pubmed-meshheading:10669310-Cannabinoids,
pubmed-meshheading:10669310-Chromatography, High Pressure Liquid,
pubmed-meshheading:10669310-Endocannabinoids,
pubmed-meshheading:10669310-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:10669310-Glycerides,
pubmed-meshheading:10669310-Indicators and Reagents,
pubmed-meshheading:10669310-Kidney,
pubmed-meshheading:10669310-Liver,
pubmed-meshheading:10669310-Male,
pubmed-meshheading:10669310-Myocardium,
pubmed-meshheading:10669310-Neurotransmitter Agents,
pubmed-meshheading:10669310-Polyunsaturated Alkamides,
pubmed-meshheading:10669310-Rats,
pubmed-meshheading:10669310-Rats, Zucker,
pubmed-meshheading:10669310-Sensitivity and Specificity,
pubmed-meshheading:10669310-Spleen,
pubmed-meshheading:10669310-Testis
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pubmed:year |
2000
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pubmed:articleTitle |
A sensitive endocannabinoid assay. The simultaneous analysis of N-acylethanolamines and 2-monoacylglycerols.
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pubmed:affiliation |
The Hormel Institute, University of Minnesota, Austin 55912, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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