| pubmed-article:10668923 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10668923 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:10668923 | lifeskim:mentions | umls-concept:C0004358 | lld:lifeskim |
| pubmed-article:10668923 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:10668923 | lifeskim:mentions | umls-concept:C1419119 | lld:lifeskim |
| pubmed-article:10668923 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:10668923 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:10668923 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:10668923 | pubmed:dateCreated | 2000-2-29 | lld:pubmed |
| pubmed-article:10668923 | pubmed:abstractText | The tyrosine phosphatase like protein IA-2 is an important autoantigen in insulin-dependent diabetes mellitus (type 1 diabetes). Autoantibodies to IA-2 (IA-2A) are present in the serum of patients with type 1 diabetes even before the onset of the disease. Previously, we reported on a radioimmune assay to detect IA-2A, using E. coli-derived 125I-labelled IA-2 as antigen. Although this assay could be shown to be equivalent to the common reference method for IA-2A detection (radioligand assays using in vitro synthesised 35S-methionine labelled antigen), the disadvantages of both assays with respect to synthesis and handling of the radioactive antigen limit their use in routine laboratories. In this study, we have evaluated a non-radioactive enzyme-linked immunosorbent assay (ELISA) for the simple detection of IA-2A. We report on an ELISA where the biotinylated intracytoplasmic part of IA-2 (IA-2ic) is captured on streptavidin-coated plates. The sensitivity of the ELISA was similar to the validated radioligand assay, as it detected 47 of 69 (69%) patients with type 1 diabetes as compared to 46 of 68 (67 %) with the reference method for IA-2A detection (radioligand assays using in vitro synthesised 35S-methionine labelled antigen). Only 2 of 50 (4%) patients with autoimmune thyroid disease and 1 of 114 (1 %) healthy controls were detected in the ELISA, confirming specificity. There was a significant correlation between the ELISA and the radioligand assay (r = 0.64, p<0.001). We conclude that this ELISA is suitable to detect IA-2A in the serum of patients with type 1 diabetes with a similar sensitivity and specificity to the radioligand assay. This ELISA will allow rapid and simple measurement of IA-2A where the radioligand assay is inconvenient or not available. | lld:pubmed |
| pubmed-article:10668923 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10668923 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10668923 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10668923 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10668923 | pubmed:issn | 0018-5043 | lld:pubmed |
| pubmed-article:10668923 | pubmed:author | pubmed-author:ScherbaumW... | lld:pubmed |
| pubmed-article:10668923 | pubmed:author | pubmed-author:SeisslerJJ | lld:pubmed |
| pubmed-article:10668923 | pubmed:author | pubmed-author:MorgenthalerN... | lld:pubmed |
| pubmed-article:10668923 | pubmed:author | pubmed-author:LöbnerKK | lld:pubmed |
| pubmed-article:10668923 | pubmed:author | pubmed-author:Khoo-Morgenth... | lld:pubmed |
| pubmed-article:10668923 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10668923 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:10668923 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10668923 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10668923 | pubmed:pagination | 686-91 | lld:pubmed |
| pubmed-article:10668923 | pubmed:dateRevised | 2009-2-19 | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
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| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
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| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:meshHeading | pubmed-meshheading:10668923... | lld:pubmed |
| pubmed-article:10668923 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10668923 | pubmed:articleTitle | Detection of autoantibodies to the diabetes-associated antigen IA-2 by a sensitive enzyme-linked immunosorbent assay. | lld:pubmed |
| pubmed-article:10668923 | pubmed:affiliation | Department of Internal Medicine III, University of Leipzig, Germany. | lld:pubmed |
| pubmed-article:10668923 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10668923 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:10668923 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
