Linked Life Data

10668923

Source:http://linkedlifedata.com/resource/pubmed/id/10668923

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2000-2-29
pubmed:abstractText
The tyrosine phosphatase like protein IA-2 is an important autoantigen in insulin-dependent diabetes mellitus (type 1 diabetes). Autoantibodies to IA-2 (IA-2A) are present in the serum of patients with type 1 diabetes even before the onset of the disease. Previously, we reported on a radioimmune assay to detect IA-2A, using E. coli-derived 125I-labelled IA-2 as antigen. Although this assay could be shown to be equivalent to the common reference method for IA-2A detection (radioligand assays using in vitro synthesised 35S-methionine labelled antigen), the disadvantages of both assays with respect to synthesis and handling of the radioactive antigen limit their use in routine laboratories. In this study, we have evaluated a non-radioactive enzyme-linked immunosorbent assay (ELISA) for the simple detection of IA-2A. We report on an ELISA where the biotinylated intracytoplasmic part of IA-2 (IA-2ic) is captured on streptavidin-coated plates. The sensitivity of the ELISA was similar to the validated radioligand assay, as it detected 47 of 69 (69%) patients with type 1 diabetes as compared to 46 of 68 (67 %) with the reference method for IA-2A detection (radioligand assays using in vitro synthesised 35S-methionine labelled antigen). Only 2 of 50 (4%) patients with autoimmune thyroid disease and 1 of 114 (1 %) healthy controls were detected in the ELISA, confirming specificity. There was a significant correlation between the ELISA and the radioligand assay (r = 0.64, p<0.001). We conclude that this ELISA is suitable to detect IA-2A in the serum of patients with type 1 diabetes with a similar sensitivity and specificity to the radioligand assay. This ELISA will allow rapid and simple measurement of IA-2A where the radioligand assay is inconvenient or not available.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0018-5043
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
686-91
pubmed:dateRevised
2009-2-19
pubmed:meshHeading
pubmed-meshheading:10668923-Antibody Specificity, pubmed-meshheading:10668923-Autoantibodies, pubmed-meshheading:10668923-Autoantigens, pubmed-meshheading:10668923-Blotting, Western, pubmed-meshheading:10668923-Diabetes Mellitus, Type 1, pubmed-meshheading:10668923-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:10668923-Humans, pubmed-meshheading:10668923-Immunoblotting, pubmed-meshheading:10668923-Membrane Proteins, pubmed-meshheading:10668923-Protein Tyrosine Phosphatase, Non-Receptor Type 1, pubmed-meshheading:10668923-Protein Tyrosine Phosphatases, pubmed-meshheading:10668923-Radioligand Assay, pubmed-meshheading:10668923-Receptor-Like Protein Tyrosine Phosphatases, Class 8, pubmed-meshheading:10668923-Recombinant Fusion Proteins, pubmed-meshheading:10668923-Sensitivity and Specificity, pubmed-meshheading:10668923-Streptavidin, pubmed-meshheading:10668923-Sulfur Radioisotopes
pubmed:year
1999
pubmed:articleTitle
Detection of autoantibodies to the diabetes-associated antigen IA-2 by a sensitive enzyme-linked immunosorbent assay.
pubmed:affiliation
Department of Internal Medicine III, University of Leipzig, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't