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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-3-21
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pubmed:abstractText |
Calcium influx and elevation of intracellular free calcium ([Ca2+]i), with subsequent activation of degradative enzymes, is hypothesized to cause cell injury and death after traumatic brain injury. We examined the effects of mild-to-severe stretch-induced traumatic injury on [Ca2+]i dynamics in cortical neurons cultured on silastic membranes. [Ca2+]i was rapidly elevated after injury, however, the increase was transient with neuronal [Ca2+]i returning to basal levels by 3 h after injury, except in the most severely injured cells. Despite a return of [Ca2+]i to basal levels, there were persistent alterations in calcium-mediated signal transduction through 24 h after injury. [Ca2+]i elevation in response to glutamate or NMDA was enhanced after injury. We also found novel alterations in intracellular calcium store-mediated signaling. Neuronal calcium stores failed to respond to a stimulus 15 min after injury and exhibited potentiated responses to stimuli at 3 and 24 h post-injury. Thus, changes in calcium-mediated cellular signaling may contribute to the pathology that is observed after traumatic brain injury.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0143-4160
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
289-99
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10668567-Animals,
pubmed-meshheading:10668567-Brain Injuries,
pubmed-meshheading:10668567-Calcium,
pubmed-meshheading:10668567-Calcium Signaling,
pubmed-meshheading:10668567-Cells, Cultured,
pubmed-meshheading:10668567-Cerebral Cortex,
pubmed-meshheading:10668567-Cycloleucine,
pubmed-meshheading:10668567-Enzyme Inhibitors,
pubmed-meshheading:10668567-Excitatory Amino Acid Agonists,
pubmed-meshheading:10668567-Glutamic Acid,
pubmed-meshheading:10668567-N-Methylaspartate,
pubmed-meshheading:10668567-Neuroglia,
pubmed-meshheading:10668567-Neurons,
pubmed-meshheading:10668567-Neuroprotective Agents,
pubmed-meshheading:10668567-Rats,
pubmed-meshheading:10668567-Rats, Sprague-Dawley,
pubmed-meshheading:10668567-Thapsigargin
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pubmed:year |
1999
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pubmed:articleTitle |
Alterations in calcium-mediated signal transduction after traumatic injury of cortical neurons.
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pubmed:affiliation |
Department of Pharmacology and Toxicology, Medical College of Virginia of Virginia Commonwealth University, Richmond, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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