Source:http://linkedlifedata.com/resource/pubmed/id/10666577
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 12
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pubmed:dateCreated |
2000-3-17
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pubmed:abstractText |
The crystallization and structure determination of recombinant human apolipoprotein A-I (apo A-I), the major protein component of high-density lipoprotein, is described. The fragment crystallized, residues 44-243 of native apo A-I [apo Delta(1-43)A-I], is very similar to intact native apo A-I in its ability to bind lipid, to be incorporated into high-density lipoproteins and to activate lecithin-cholesterol acyl transferase. Apo Delta(1-43)A-I crystallizes from 1.0-1.4 M sodium citrate pH 6.5-7.5 in space group P2(1)2(1)2(1), with unit-cell parameters a = 97.47, b = 113.87, c = 196.19 A (crystal form I). The crystals exhibit unusual diffraction intensity spikes and axial extinctions that are discussed in the context of the 4 A crystal structure. When flash-cooled to 100 K, the crystals diffract synchrotron radiation to 3 A resolution. Radiation sensitivity and crystal-to-crystal variation have hindered the assembly of a complete 3 A data set.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0907-4449
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2013-21
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10666577-Apolipoprotein A-I,
pubmed-meshheading:10666577-Crystallization,
pubmed-meshheading:10666577-Crystallography, X-Ray,
pubmed-meshheading:10666577-Humans,
pubmed-meshheading:10666577-Models, Molecular,
pubmed-meshheading:10666577-Peptide Fragments,
pubmed-meshheading:10666577-Recombinant Proteins,
pubmed-meshheading:10666577-Static Electricity
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pubmed:year |
1999
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pubmed:articleTitle |
Human apolipoprotein A-I: structure determination and analysis of unusual diffraction characteristics.
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pubmed:affiliation |
Department of Organic Chemistry, Southern Research Institute, Birmingham, AL 35205, USA. borhani@sri.org
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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