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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2000-3-14
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pubmed:abstractText |
Previously, we demonstrated that B-chronic lymphocytic leukemia (B-CLL) cells could be divided into 2 groups depending on the expression of CD38 by the malignant cells. The 2 groups differed in their signal-transducing capacities initiated by cross-linking of surface IgM; only in CD38-positive cells was an efficient signal delivered, invariably resulting in cell apoptosis. In this study, we investigated the effect of surface IgD cross-linking in 10 patients with CD38-positive B-CLL. Exposure of the malignant cells to goat antihuman delta-chain antibodies (Gadelta-ab) caused [Ca(++)]i mobilization and tyrosine kinase phosphorylation in a manner not different from that observed after goat antihuman mu-chain antibody (Gamu-ab) treatment in vitro. However, Gadelta-ab-treated cells failed to undergo apoptosis and instead displayed prolonged survival in culture and differentiated into plasma cells when rIL2 was concomitantly present. Cross-linking of surface IgD failed to induce proliferation of the malignant cells in vitro. Moreover, treatment with Gadelta-ab did not prevent apoptosis of B-CLL cells induced by Gamu-ab. Collectively, these experiments demonstrated that IgM and IgD expressed by the same cell may deliver opposite signals under particular circumstances and provide some clues for the understanding of the pathophysiology of B-CLL. (Blood. 2000;95:1199-1206)
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin D,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin gamma-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/NAD Nucleosidase,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1199-206
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10666191-ADP-ribosyl Cyclase,
pubmed-meshheading:10666191-Antibodies,
pubmed-meshheading:10666191-Antigens, CD,
pubmed-meshheading:10666191-Antigens, CD38,
pubmed-meshheading:10666191-Antigens, Differentiation,
pubmed-meshheading:10666191-Apoptosis,
pubmed-meshheading:10666191-B-Lymphocytes,
pubmed-meshheading:10666191-Calcium,
pubmed-meshheading:10666191-Cell Cycle,
pubmed-meshheading:10666191-Cell Differentiation,
pubmed-meshheading:10666191-Cross-Linking Reagents,
pubmed-meshheading:10666191-Flow Cytometry,
pubmed-meshheading:10666191-Humans,
pubmed-meshheading:10666191-Immunoglobulin D,
pubmed-meshheading:10666191-Immunoglobulin M,
pubmed-meshheading:10666191-Immunoglobulin gamma-Chains,
pubmed-meshheading:10666191-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:10666191-Lymphocyte Activation,
pubmed-meshheading:10666191-Membrane Glycoproteins,
pubmed-meshheading:10666191-NAD+ Nucleosidase,
pubmed-meshheading:10666191-Necrosis,
pubmed-meshheading:10666191-Phosphotyrosine,
pubmed-meshheading:10666191-Recombinant Proteins,
pubmed-meshheading:10666191-Signal Transduction,
pubmed-meshheading:10666191-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
Apoptosis or plasma cell differentiation of CD38-positive B-chronic lymphocytic leukemia cells induced by cross-linking of surface IgM or IgD.
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pubmed:affiliation |
Servizio di Immunologia Clinica, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy. szupo@hp380.ist.unige.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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