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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-4-14
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pubmed:abstractText |
Polyamines are required for the proliferation of the rat intestinal mucosal IEC-6 cell line. Ornithine decarboxylase (ODC) is the enzyme that catalyzes the first step in polyamine synthesis. ODC inhibition not only leads to polyamine depletion but also leads to inhibition of cell proliferation and regulates the expression of the immediate-early genes c-fos, c-myc, and c-jun. Members of the signal transducers and activators of transcription (STAT) transcription factor family bind to the sis-inducible element (SIE) present in the promoters to regulate the expression of a variety of important genes. In the present study, we tested the hypothesis that the STAT3 transcription factor, which is responsible for activation of the acute phase response genes, is activated after inhibition of ODC. We found that inhibition of ODC rapidly induces STAT3 activation as determined by STAT3 tyrosine phosphorylation, translocation of STAT3 from the cytoplasm into the nucleus, and the presence of STAT3 in SIE-dependent DNA-protein complexes. STAT3 activation upon inhibition of ODC was accompanied by the activation of a STAT3-dependent reporter construct. Moreover, prolonged polyamine depletion resulted in downregulation of cellular STAT3 levels.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Choline O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Eflornithine,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Ornithine Decarboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Polyamines,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0363-6143
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
C331-5
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10666028-Animals,
pubmed-meshheading:10666028-Cell Line,
pubmed-meshheading:10666028-Cell Nucleus,
pubmed-meshheading:10666028-Choline O-Acetyltransferase,
pubmed-meshheading:10666028-Cytoplasm,
pubmed-meshheading:10666028-DNA Probes,
pubmed-meshheading:10666028-DNA-Binding Proteins,
pubmed-meshheading:10666028-Eflornithine,
pubmed-meshheading:10666028-Enzyme Inhibitors,
pubmed-meshheading:10666028-Epithelial Cells,
pubmed-meshheading:10666028-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10666028-Genes, Reporter,
pubmed-meshheading:10666028-Intestines,
pubmed-meshheading:10666028-Ornithine Decarboxylase,
pubmed-meshheading:10666028-Phosphorylation,
pubmed-meshheading:10666028-Polyamines,
pubmed-meshheading:10666028-Promoter Regions, Genetic,
pubmed-meshheading:10666028-Rats,
pubmed-meshheading:10666028-STAT3 Transcription Factor,
pubmed-meshheading:10666028-Signal Transduction,
pubmed-meshheading:10666028-Trans-Activators,
pubmed-meshheading:10666028-Transcriptional Activation,
pubmed-meshheading:10666028-Tyrosine
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pubmed:year |
2000
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pubmed:articleTitle |
Inhibition of ornithine decarboxylase induces STAT3 tyrosine phosphorylation and DNA binding in IEC-6 cells.
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pubmed:affiliation |
Department of Pathology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA. lpfeffer@utmem.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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