Source:http://linkedlifedata.com/resource/pubmed/id/10665827
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2000-2-18
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pubmed:abstractText |
Previous studies in vitro have shown that NK3 receptors exist on primary afferent terminals in rat spinal cord and mediate potentiation of the depolarisation-evoked substance P (SP) release. In the present study we have investigated the role of the NK3 receptor-mediated SP release system in a model of inflammatory pain. Monoarthritis was induced in rats by unilateral injection of complete Freund's adjuvant (CFA); withdrawal latencies to a thermal stimulus were subsequently measured at various times following CFA. The CFA-treated paw displayed hyperalgesia as early as 4 h after CFA injection and hyperalgesia was maintained until day 4 but had disappeared by day 21. The thermal hyperalgesia was associated with an increase in basal SP release from spinal cord synaptosomes. The possible involvement of endogenous neurokinin B acting at NK3 receptors was tested by using SB 223412-A [(S)-(-)-N-(alpha-ethylbenzyl)-3-hydroxy-2-phenylquinoline-4-carbo xamide hydrochloride], a novel, potent (Ki=30 nM) and selective (Ki>10,000 nM for NK1 and NK2 receptors), non-peptidic NK3 receptor antagonist. In vitro SB 223412-A antagonised the potentiation of SP release produced by senktide in spinal cord synaptosomes. Administered systemically to monoarthritic rats (50 mg/kg, p.o., b.i.d., for 4 days), the NK3 receptor antagonist SB 223412-A significantly reduced thermal hyperalgesia and normalised the basal release of SP from spinal cord synaptosomes. The data suggest that neurokinin B acting at NK3 receptors that mediate SP release within the spinal cord play a role in inflammation. These NK3 receptors may represent, therefore, appropriate targets in the therapy of inflammatory pain.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Freund's Adjuvant,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Quinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurokinin-3,
http://linkedlifedata.com/resource/pubmed/chemical/SB 223412,
http://linkedlifedata.com/resource/pubmed/chemical/Substance P,
http://linkedlifedata.com/resource/pubmed/chemical/senktide
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pubmed:status |
MEDLINE
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pubmed:issn |
0028-3908
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
141-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10665827-Afferent Pathways,
pubmed-meshheading:10665827-Animals,
pubmed-meshheading:10665827-Arthritis, Experimental,
pubmed-meshheading:10665827-Freund's Adjuvant,
pubmed-meshheading:10665827-Hyperalgesia,
pubmed-meshheading:10665827-Inflammation,
pubmed-meshheading:10665827-Male,
pubmed-meshheading:10665827-Pain,
pubmed-meshheading:10665827-Peptide Fragments,
pubmed-meshheading:10665827-Quinolines,
pubmed-meshheading:10665827-Rats,
pubmed-meshheading:10665827-Rats, Sprague-Dawley,
pubmed-meshheading:10665827-Reaction Time,
pubmed-meshheading:10665827-Receptors, Neurokinin-3,
pubmed-meshheading:10665827-Spinal Cord,
pubmed-meshheading:10665827-Substance P
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pubmed:year |
2000
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pubmed:articleTitle |
NK3 receptor blockade prevents hyperalgesia and the associated spinal cord substance P release in monoarthritic rats.
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pubmed:affiliation |
Department of Biology, SmithKline Beecham S.p.A., Milan, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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