rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1
|
pubmed:dateCreated |
2000-3-24
|
pubmed:abstractText |
For the potential use as recombinant vaccine, canine parvovirus (CPV) major capsid protein VP2 was expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) vector. CPV VP2 gene was introduced into polyhedrin-based BmNPV transfer vector pBmKSK3, and recombinant virus BmK1-Parvo was prepared. When anti-CPV.VP2 monoclonal antibody was employed in immunofluorescence staining, an intense signal was observed within BmK1-Parvo-infected Bm5 cells but not within uninfected cells or cells infected with a wild-type BmNPV-K1. In hemagglutination assay, the expression level of VP2 were 3.2 x 10(3) HA units/ml from infected Bm5 cells, 2.1x 10(5) HA units/larvae from infected larval fat body, and 1.6x 10(6) HA units/ml from infected larval hemolymph. These results suggested that BmNPV vector system using B. mori larva as host could be applied to efficient mass-production of recombinant vaccines.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:issn |
0304-8608
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
145
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
171-7
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:10664415-Animals,
pubmed-meshheading:10664415-Bombyx,
pubmed-meshheading:10664415-Capsid,
pubmed-meshheading:10664415-Capsid Proteins,
pubmed-meshheading:10664415-Cells, Cultured,
pubmed-meshheading:10664415-DNA, Recombinant,
pubmed-meshheading:10664415-Dogs,
pubmed-meshheading:10664415-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:10664415-Genetic Vectors,
pubmed-meshheading:10664415-Hemagglutination Tests,
pubmed-meshheading:10664415-Hemolymph,
pubmed-meshheading:10664415-Nucleopolyhedrovirus,
pubmed-meshheading:10664415-Parvovirus, Canine,
pubmed-meshheading:10664415-Promoter Regions, Genetic,
pubmed-meshheading:10664415-Recombinant Proteins,
pubmed-meshheading:10664415-Viral Proteins,
pubmed-meshheading:10664415-Viral Structural Proteins
|
pubmed:year |
2000
|
pubmed:articleTitle |
High-level expression of canine parvovirus VP2 using Bombyx mori nucleopolyhedrovirus vector.
|
pubmed:affiliation |
Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|