Linked Life Data

10662852

Source:http://linkedlifedata.com/resource/pubmed/id/10662852

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-2-25
pubmed:abstractText
Neurotransmitter synthesis is regulated by a variety of factors, yet the effect of altering transmitter content on the operation of neuronal circuits has been relatively unexplored. We used electrophysiological, electrochemical, and immunohistochemical techniques to investigate the effects of augmenting the serotonin (5-HT) content of identified serotonergic neurons embedded in a simple motor circuit. The dorsal swim interneurons (DSIs) are serotonergic neurons intrinsic to the central pattern generator (CPG) for swimming in the mollusc Tritonia diomedea. As expected, treatment with the serotonin precursor 5-hydroxytryptophan (5-HTP) increased the intensity of serotonin immunolabeling and enhanced the potency of synaptic and modulatory actions elicited by the DSIs. It also greatly enhanced the ability of the DSIs to evoke rhythmic CPG activity. After 5-HTP treatment, microvoltammetric measurements indicated an increase in a putative 5-HT electrochemical signal during swim CPG activation. Paradoxically, the spiking activity of the serotonergic neurons decreased to a single burst at the onset of the rhythmic motor program, whereas the overall duration of the episode remained about the same. 5-HTP treatment gradually reduced the rhythmicity of the CPG output. Thus, more serotonin did not result in a more robust swim motor program, suggesting that serotonin synthesis must be kept within certain limits for the circuit to function correctly and indicating that altering neurotransmitter synthesis can have serious consequences for the output of neural networks.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1622-34
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Paradoxical actions of the serotonin precursor 5-hydroxytryptophan on the activity of identified serotonergic neurons in a simple motor circuit.
pubmed:affiliation
Department of Biology, Georgia State University, Atlanta, Georgia 30302, USA. biodjf@panther.gsu.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.