Linked Life Data

10662503

Source:http://linkedlifedata.com/resource/pubmed/id/10662503

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-3-14
pubmed:abstractText
Specification and polarization of the midbrain and anterior hindbrain involve planar signals originating from the isthmus. Current evidence suggests that FGF8, expressed at the isthmus, provides this patterning influence. In this study, we have sought to identify novel genes which are involved in the process by which regional identity is imparted to midbrain and anterior hindbrain (rhombomere 1). An enhanced differential display reverse transcription method was used to clone cDNAs derived from transcripts expressed specifically in either rhombomere 1 or midbrain during the period of isthmic patterning activity. This gene expression screen identified 28 differentially expressed cDNAs. A clone upregulated in cDNA derived from rhombomere 1 tissue showed a 91% identity at the nucleotide level to the putative human receptor tyrosine kinase antagonist: sprouty2. In situ hybridization on whole chick embryos showed chick sprouty2 to be expressed initially within the isthmus and rhombomere 1, spatially and temporally coincident with Fgf8 expression. However, at later stages this domain was more extensive than that of Fgf8. Introduction of ligand-coated beads into either midbrain or hindbrain region revealed that sprouty2 could be rapidly induced by FGF8. These data suggest that sprouty2 participates in a negative feedback regulatory loop to modulate the patterning activity of FGF8 at the isthmus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1044-7431
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
22-35
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10662503-Amino Acid Sequence, pubmed-meshheading:10662503-Animals, pubmed-meshheading:10662503-Body Patterning, pubmed-meshheading:10662503-Chick Embryo, pubmed-meshheading:10662503-Chickens, pubmed-meshheading:10662503-DNA, Complementary, pubmed-meshheading:10662503-Drosophila, pubmed-meshheading:10662503-Fibroblast Growth Factor 8, pubmed-meshheading:10662503-Fibroblast Growth Factors, pubmed-meshheading:10662503-Gene Expression Regulation, Developmental, pubmed-meshheading:10662503-Humans, pubmed-meshheading:10662503-Mesencephalon, pubmed-meshheading:10662503-Mice, pubmed-meshheading:10662503-Molecular Sequence Data, pubmed-meshheading:10662503-Nerve Tissue Proteins, pubmed-meshheading:10662503-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:10662503-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10662503-Rhombencephalon, pubmed-meshheading:10662503-Sequence Alignment, pubmed-meshheading:10662503-Sequence Homology, Amino Acid, pubmed-meshheading:10662503-Transcription, Genetic
pubmed:year
2000
pubmed:articleTitle
Differential display of genes expressed at the midbrain - hindbrain junction identifies sprouty2: an FGF8-inducible member of a family of intracellular FGF antagonists.
pubmed:affiliation
MRC Brain Development Programme, Centre for Developmental Neurobiology, King's College London, New Hunt's House, Guy's Campus, London, SE1 9RT, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't