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rdf:type |
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lifeskim:mentions |
umls-concept:C0025186,
umls-concept:C0162610,
umls-concept:C0205197,
umls-concept:C0290178,
umls-concept:C1335227,
umls-concept:C1514873,
umls-concept:C1523105,
umls-concept:C1546857,
umls-concept:C1554962,
umls-concept:C1556066,
umls-concept:C1619636
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pubmed:issue |
1
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pubmed:dateCreated |
2000-5-9
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pubmed:abstractText |
The Polo-like kinases are key regulatory molecules required during the cell cycle for the successful completion of mitosis. We have cloned a C. elegans homolog of the Drosophila melanogaster polo gene (designated plk-1 for C. elegans polo-like kinase-1) and present the subcellular localization of the PLK-1 protein during the meiotic and mitotic cell cycles in C. elegans oocytes and embryos, respectively. Disruption of PLK-1 expression by RNA-mediated interference (RNAi) disrupts normal oocyte and embryonic development. Inspection of oocytes revealed a defect in nuclear envelope breakdown (NEBD) before ovulation. This defect in NEBD was also observed in oocytes that were depleted of the cyclin-dependent kinase NCC-1 (C. elegans homolog of Cdc2). The plk-1 RNAi oocytes were fertilized; however the resulting embryos were unable to separate their meiotic chromosomes or form and extrude polar bodies. These defects led to embryonic arrest as single cells. genesis 26:26-41, 2000. Published 2000 Wiley-Liss, Inc.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Helminth Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Double-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/ncc-1 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/polo-like kinase 1
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1526-954X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
26-41
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:10660671-Amino Acid Sequence,
pubmed-meshheading:10660671-Animals,
pubmed-meshheading:10660671-Base Sequence,
pubmed-meshheading:10660671-Caenorhabditis elegans,
pubmed-meshheading:10660671-Caenorhabditis elegans Proteins,
pubmed-meshheading:10660671-Cell Cycle Proteins,
pubmed-meshheading:10660671-DNA, Complementary,
pubmed-meshheading:10660671-Embryo, Nonmammalian,
pubmed-meshheading:10660671-Enzyme Induction,
pubmed-meshheading:10660671-Gene Expression Regulation, Developmental,
pubmed-meshheading:10660671-Genes, Helminth,
pubmed-meshheading:10660671-Helminth Proteins,
pubmed-meshheading:10660671-Meiosis,
pubmed-meshheading:10660671-Mitosis,
pubmed-meshheading:10660671-Molecular Sequence Data,
pubmed-meshheading:10660671-Nuclear Envelope,
pubmed-meshheading:10660671-Oocytes,
pubmed-meshheading:10660671-Protein Kinases,
pubmed-meshheading:10660671-Protein-Serine-Threonine Kinases,
pubmed-meshheading:10660671-Proto-Oncogene Proteins,
pubmed-meshheading:10660671-RNA, Double-Stranded,
pubmed-meshheading:10660671-Sequence Alignment,
pubmed-meshheading:10660671-Sequence Homology, Amino Acid
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pubmed:year |
2000
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pubmed:articleTitle |
The polo-like kinase PLK-1 is required for nuclear envelope breakdown and the completion of meiosis in Caenorhabditis elegans.
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pubmed:affiliation |
National Institute on Aging, National Cancer Institute-FCRF, Frederick, Maryland 21702, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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