|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2000-3-16
|
|
pubmed:abstractText |
Treatment of dopaminergic rat PC12 cells with human immunodeficiency virus, type 1 (HIV-1) Tat protein or tat cDNA inhibited the expression of tyrosine hydroxylase (TH), the rate-limiting enzyme for the dopamine biosynthetic pathway, as well as the production and release of dopamine into the culture medium. Moreover, the Tat addition to PC12 cells up-regulated the expression of the inducible cAMP early repressor (ICER), a specific member of the cAMP-responsive element modulator transcription factor family, in a cAMP-dependent manner. In turn, ICER overexpression abrogated the transcription activity of the TH promoter in PC12 cells, strongly suggesting ICER involvement in Tat-mediated inhibition of TH gene expression. In vivo injection of synthetic HIV-1 Tat protein into the striatum of healthy rats induced a subclinical Parkinson's-like disease that became manifested only when the animals were treated with amphetamine. As early as one week postinjection, the histochemical examination of the rat substantia nigra showed a reduced staining of neurons expressing TH followed by a loss of TH(+) neurons at later time points. As Tat protein can be locally released into the central nervous system by HIV-1-infected microglial cells, our findings may contribute to the explanation of the pathogenesis of the motorial abnormalities often reported in HIV-1 seropositive individuals.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response Element...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidopamine,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
11
|
|
pubmed:volume |
275
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4159-65
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:10660577-Animals,
pubmed-meshheading:10660577-Behavior, Animal,
pubmed-meshheading:10660577-Cyclic AMP,
pubmed-meshheading:10660577-Cyclic AMP Response Element Modulator,
pubmed-meshheading:10660577-DNA-Binding Proteins,
pubmed-meshheading:10660577-Dopamine,
pubmed-meshheading:10660577-Forskolin,
pubmed-meshheading:10660577-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10660577-Gene Products, tat,
pubmed-meshheading:10660577-HIV-1,
pubmed-meshheading:10660577-Humans,
pubmed-meshheading:10660577-Male,
pubmed-meshheading:10660577-Mesencephalon,
pubmed-meshheading:10660577-Microscopy, Electron,
pubmed-meshheading:10660577-Oxidopamine,
pubmed-meshheading:10660577-PC12 Cells,
pubmed-meshheading:10660577-RNA, Messenger,
pubmed-meshheading:10660577-Rats,
pubmed-meshheading:10660577-Rats, Wistar,
pubmed-meshheading:10660577-Repressor Proteins,
pubmed-meshheading:10660577-Tyrosine 3-Monooxygenase,
pubmed-meshheading:10660577-tat Gene Products, Human Immunodeficiency Virus
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
HIV-1 Tat-mediated inhibition of the tyrosine hydroxylase gene expression in dopaminergic neuronal cells.
|
|
pubmed:affiliation |
Department of Morphology and Embryology, Anatomy Section, University of Ferrara, Via Fossato di Mortara 66, 44100 Ferrara, Italy. zlg@dns.unife.it
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
