| pubmed-article:10660569 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10660569 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:10660569 | lifeskim:mentions | umls-concept:C0082329 | lld:lifeskim |
| pubmed-article:10660569 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
| pubmed-article:10660569 | lifeskim:mentions | umls-concept:C0002085 | lld:lifeskim |
| pubmed-article:10660569 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:10660569 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:10660569 | pubmed:dateCreated | 2000-3-16 | lld:pubmed |
| pubmed-article:10660569 | pubmed:abstractText | In order to define regions of ParE, one of the two subunits of topoisomerase IV, that are involved in catalysis during topoisomerization, we developed a selection procedure to isolate dominant-negative parE alleles. Both wild-type parC and mutagenized parE were expressed from a tightly-regulated lac promoter on a moderate-copy plasmid. Mutated parE alleles were rescued from those plasmids that caused IPTG-dependent cell death. The mutant ParE proteins could be divided into two groups when reconstituted with ParC to form topoisomerase IV, those that elicited hyper-DNA cleavage and those that affected covalent complex formation. | lld:pubmed |
| pubmed-article:10660569 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10660569 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10660569 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10660569 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10660569 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10660569 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10660569 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10660569 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10660569 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10660569 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10660569 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10660569 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10660569 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:10660569 | pubmed:author | pubmed-author:MariansK JKJ | lld:pubmed |
| pubmed-article:10660569 | pubmed:author | pubmed-author:LevineCC | lld:pubmed |
| pubmed-article:10660569 | pubmed:author | pubmed-author:NursePP | lld:pubmed |
| pubmed-article:10660569 | pubmed:author | pubmed-author:PeneJJ | lld:pubmed |
| pubmed-article:10660569 | pubmed:author | pubmed-author:BahngSS | lld:pubmed |
| pubmed-article:10660569 | pubmed:author | pubmed-author:MossessovaEE | lld:pubmed |
| pubmed-article:10660569 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10660569 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:10660569 | pubmed:volume | 275 | lld:pubmed |
| pubmed-article:10660569 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10660569 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10660569 | pubmed:pagination | 4099-103 | lld:pubmed |
| pubmed-article:10660569 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
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| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
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| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
| pubmed-article:10660569 | pubmed:meshHeading | pubmed-meshheading:10660569... | lld:pubmed |
| pubmed-article:10660569 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10660569 | pubmed:articleTitle | Mutational analysis of Escherichia coli topoisomerase IV. I. Selection of dominant-negative parE alleles. | lld:pubmed |
| pubmed-article:10660569 | pubmed:affiliation | Molecular Biology Graduate Program, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA. | lld:pubmed |
| pubmed-article:10660569 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10660569 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| entrez-gene:947501 | entrezgene:pubmed | pubmed-article:10660569 | lld:entrezgene |
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