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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-3-16
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pubmed:abstractText |
In order to define regions of ParE, one of the two subunits of topoisomerase IV, that are involved in catalysis during topoisomerization, we developed a selection procedure to isolate dominant-negative parE alleles. Both wild-type parC and mutagenized parE were expressed from a tightly-regulated lac promoter on a moderate-copy plasmid. Mutated parE alleles were rescued from those plasmids that caused IPTG-dependent cell death. The mutant ParE proteins could be divided into two groups when reconstituted with ParC to form topoisomerase IV, those that elicited hyper-DNA cleavage and those that affected covalent complex formation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4099-103
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10660569-Alleles,
pubmed-meshheading:10660569-Bacterial Proteins,
pubmed-meshheading:10660569-DNA, Superhelical,
pubmed-meshheading:10660569-DNA Topoisomerase IV,
pubmed-meshheading:10660569-DNA Topoisomerases, Type II,
pubmed-meshheading:10660569-DNA-Binding Proteins,
pubmed-meshheading:10660569-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10660569-Escherichia coli,
pubmed-meshheading:10660569-Isopropyl Thiogalactoside,
pubmed-meshheading:10660569-Microscopy, Fluorescence,
pubmed-meshheading:10660569-Mutation,
pubmed-meshheading:10660569-Phenotype
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pubmed:year |
2000
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pubmed:articleTitle |
Mutational analysis of Escherichia coli topoisomerase IV. I. Selection of dominant-negative parE alleles.
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pubmed:affiliation |
Molecular Biology Graduate Program, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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