Source:http://linkedlifedata.com/resource/pubmed/id/10659793
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2000-4-14
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pubmed:databankReference | |
pubmed:abstractText |
The grain softness proteins or friabilins are known to be composed of three main components: puroindoline a, puroindoline b, and GSP-1. cDNAs for GSP-1 have previously been mapped to group-5 chromosomes and their location on chromosome 5D is closely linked to the grain hardness (Ha) locus of hexaploid wheat. A genomic DNA clone containing the GSP-1 gene (wGSP1-A1) from hexaploid wheat has been identified by fluorescent in situ hybridization as having originated from the distal end of the short arm of chromosome 5A. A genomic clone containing the gene (wGSP1-D1) was also isolated from Aegilops tauschii, the donor of the D genome to bread wheat. There are no introns in the GSP-1 genes, and there is high sequence identity between wGSP1-A1 and wGSP1-D1 up to 1 kb 5' and 300 bp 3' to wGSP1-D1. However, regions further upstream and downstream of wGSP1-D1 share no significant sequence identity to corresponding sequences in wGSP1-A1. These regions therefore identified potentially valuable sequences for tracing the Ha locus through assaying polymorphic DNA sequences. The sequence from 300 to 500 bp 3' to wGSP1-D1 (wGSP1-D13) was mapped to the Ha locus in a mapping population. wGSP1-D13 was also tightly linked to genes for puroindoline a and puroindoline b which have been previously mapped to be at the Ha locus. In addition wGSP1-D13 was used to detect RFLPs between near isogenic soft and hard Falcon lines and in a random selection of soft and hard wheats.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0831-2796
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1242-50
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:10659793-Base Sequence,
pubmed-meshheading:10659793-Chromosome Mapping,
pubmed-meshheading:10659793-Cloning, Molecular,
pubmed-meshheading:10659793-Crosses, Genetic,
pubmed-meshheading:10659793-DNA, Plant,
pubmed-meshheading:10659793-Genes, Plant,
pubmed-meshheading:10659793-Genome, Plant,
pubmed-meshheading:10659793-Genomic Library,
pubmed-meshheading:10659793-Molecular Sequence Data,
pubmed-meshheading:10659793-Nucleic Acid Hybridization,
pubmed-meshheading:10659793-Plant Proteins,
pubmed-meshheading:10659793-Polymorphism, Genetic,
pubmed-meshheading:10659793-Triticum
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pubmed:year |
1999
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pubmed:articleTitle |
The Ha locus of wheat: identification of a polymorphic region for tracing grain hardness in crosses.
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pubmed:affiliation |
CSIRO Plant Industry, Canberra, ACT, Australia.
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pubmed:publicationType |
Journal Article
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