Source:http://linkedlifedata.com/resource/pubmed/id/10657903
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2000-2-25
|
pubmed:abstractText |
The PTEN/MMAC1 gene, located on human chromosome 10q23, has recently been implicated as a candidate tumor suppressor gene in human cancers. In the present study, 12 uterine cancer cell lines and 87 uterine cancers of various grades and histological type were analyzed for PTEN/MMAC1 gene. Three of 44 endometrial carcinoma (7%) showed no PTEN/MMAC1 mRNA expression by RT-PCR analysis. Sequencing analysis of entire coding region of PTEN/MMAC1 gene revealed mutations in three of six endometrial cancer cell lines (50%) and 17 of 44 endometrial cancer tissues (39%). In contrast, for cervical cancers, only one of six cancer cell lines (2%) showed mutation, and one of 43 cancer tissues (2%) had an abnormality. Overall, 36% of the abnormal spots were located in exon 5, 24% were in exon 8, 16% were in exon 3, and 8% were in exon 6, and single cases of abnormality were found in exons 1, 4, and 7. Our results revealed that, in total, 60% of abnormalities were clustered in exons 5 and 8. Exon 5 is a functional domain of the PEN/MMAC1 gene, and therefore, abnormalities in this region may be important for loss of PTEN/MMAC1 gene function. Finally, we found a high frequency of PTEN/MMAC1 gene abnormalities in endometrial carcinomas but a low frequency in cervical carcinomas. These findings suggest that disruption of PTEN/MMAC1 by mutation or absence of expression may contribute to the pathogenesis or neoplastic evolution in a large proportion of endometrial carcinomas but in a small proportion of cervical carcinomas.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/PTEN Phosphohydrolase,
http://linkedlifedata.com/resource/pubmed/chemical/PTEN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Monoester Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0899-1987
|
pubmed:author | |
pubmed:copyrightInfo |
Copyright 2000 Wiley-Liss, Inc.
|
pubmed:issnType |
Print
|
pubmed:volume |
27
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
110-6
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:10657903-DNA, Complementary,
pubmed-meshheading:10657903-Endometrial Neoplasms,
pubmed-meshheading:10657903-Female,
pubmed-meshheading:10657903-Genes, Neoplasm,
pubmed-meshheading:10657903-Genes, Tumor Suppressor,
pubmed-meshheading:10657903-HeLa Cells,
pubmed-meshheading:10657903-Humans,
pubmed-meshheading:10657903-PTEN Phosphohydrolase,
pubmed-meshheading:10657903-Phosphoric Monoester Hydrolases,
pubmed-meshheading:10657903-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:10657903-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10657903-Sequence Analysis, DNA,
pubmed-meshheading:10657903-Tumor Cells, Cultured,
pubmed-meshheading:10657903-Tumor Suppressor Proteins,
pubmed-meshheading:10657903-Uterine Cervical Neoplasms
|
pubmed:year |
2000
|
pubmed:articleTitle |
Abnormal structure and expression of PTEN/MMAC1 gene in human uterine cancers.
|
pubmed:affiliation |
Department of Obstetrics & Gynecoloy, Asahikawa Medical College, Asahikawa, Japan. yaginum@asahikawa-med.ac.jp
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|