Linked Life Data

10655400

Source:http://linkedlifedata.com/resource/pubmed/id/10655400

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-3-16
pubmed:abstractText
The performance of the BDProbeTec ET system (BD Biosciences, Sparks, Md.) for direct detection of Mycobacterium tuberculosis complex (MTBC) in respiratory specimens was evaluated by comparing results to those of conventional mycobacterial culture performed with the BACTEC 460 TB system and Middlebrook 7H11 biplates. Patients known to have been on antituberculous therapy were excluded from the analysis. Of 600 evaluable specimens (4 specimens were excluded from the analysis due to failure of the internal amplification control [IAC]) from 332 patients, 57 grew mycobacteria; 16 were MTBC (from 12 patients), and 41 were nontuberculous mycobacteria. Of the 16 MTBC culture-positive specimens, 12 were smear positive and 4 were smear negative. BDProbeTec ET detected 14 of the 16 MTBC culture-positive specimens, resulting in initial overall sensitivity, specificity, and positive and negative predictive values of 87.5, 99.0, 70.0, and 99.7%, respectively. After resolution of discrepancies by review of medical records and retesting of samples yielding discordant MTBC culture and BDProbeTec ET results, the revised overall sensitivity, specificity, and positive and negative predictive values of the BDProbeTec ET were respectively 93.8, 99.8, 93.8, and 99.8% by specimen and 91.7, 99.7, 91.7, and 99.7% by patient. The BDProbeTec ET System offers the distinct advantage of including an IAC in the specimen well. These data suggest that the test performance is very good, especially for smear-positive samples. However, the number of patients with tuberculosis in our study, especially those with smear-negative disease, was small; therefore, additional studies are needed.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-10203498, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-10351985, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-8041630, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-8567906, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-8727880, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-8748288, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-8748289, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-8865173, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-8922809, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-8968906, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-9154896, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-9508296, http://linkedlifedata.com/resource/pubmed/commentcorrection/10655400-9705434
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
863-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Clinical evaluation of the BDProbeTec ET system for rapid detection of Mycobacterium tuberculosis.
pubmed:affiliation
Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0740, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't