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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-3-16
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pubmed:abstractText |
The Light Cycler technique combines rapid in vitro amplification of DNA in glass capillaries with real-time species determination and quantification of DNA load. We have established a quantitative PCR protocol for two clinically important pathogens, Candida albicans and Aspergillus fumigatus. The sensitivity of the assay was comparable to those of previously described PCR protocols (5 CFU/ml). Specific detection of C. albicans and A. fumigatus could be achieved. The assay showed a high reproducibility of 96 to 99%. The assay was linear in a range between 10(1) and 10(4) Aspergillus conidia. As capillaries do not have to be reopened for post-PCR analysis, the risk of carryover contaminations could be minimized. The Light Cycler allowed quantification of the fungal loads in a limited number of clinical specimens from patients with hematological malignancies and histologically proven invasive fungal infections. Five of nine positive samples had fungal loads between 5 and 10 CFU/ml of blood, two of nine positive samples had fungal loads between 10 and 100 CFU/ml of blood, and two of nine samples had fungal loads of more than 100 CFU/ml of blood. All samples were also found to be PCR positive by PCR-enzyme-linked immunosorbent assay analysis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-10075496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-10194462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-10325351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-1871133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-2200156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-7665644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-7883883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-7989540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-8078144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-8366523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-9163443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-9564455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-9574670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-9591711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10655350-9650962
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
586-90
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10655350-Aspergillosis,
pubmed-meshheading:10655350-Aspergillus fumigatus,
pubmed-meshheading:10655350-Candida albicans,
pubmed-meshheading:10655350-Candidiasis,
pubmed-meshheading:10655350-DNA, Fungal,
pubmed-meshheading:10655350-Electrophoresis, Agar Gel,
pubmed-meshheading:10655350-Humans,
pubmed-meshheading:10655350-Polymerase Chain Reaction,
pubmed-meshheading:10655350-Reproducibility of Results,
pubmed-meshheading:10655350-Sensitivity and Specificity,
pubmed-meshheading:10655350-Spectrometry, Fluorescence,
pubmed-meshheading:10655350-Thermodynamics
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pubmed:year |
2000
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pubmed:articleTitle |
Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system.
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pubmed:affiliation |
Medizinische Klinik, Abteilung II, Eberhard-Karls-Universität, 72076 Tübingen, Germany. juergen.loeffler@med.uni-tuebingen.de
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pubmed:publicationType |
Journal Article
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