Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-2-25
pubmed:abstractText
The sequence, temperature, concentration, and solvent dependence of singlet energy transfer from normal DNA bases to the 2-aminopurine base in synthesized DNA oligomers were investigated by optical spectroscopy. Transfer was shown directly by a variable fluorescence excitation band at 260-280 nm. Adenine (A) is the most efficient energy donor by an order of magnitude. Stacks of A adjacent to 2AP act as an antenna for 2AP excitation. An interposed G, C, or T base between A and 2AP effectively blocks transfer from A to 2AP. Base stacking facilitates transfer, while base pairing reduces energy transfer slightly. The efficiency is differentially temperature dependent in single- and double-stranded oligomers and is highest below 0 degrees C in samples measured. An efficiency transition occurs well below the melting transition of a double-stranded decamer. The transfer efficiency in the duplex decamer d(CTGA[2AP]TTCAG)(2) is moderately dependent on the sample and salt concentration and is solvent dependent. Transfer at physiological temperature over more than a few bases is improbable, except along consecutive A's, indicating that singlet energy transfer is not a major factor in the localization of UV damage in DNA. These results have features in common with recently observed electron transfer from 2AP to G in oligonucleotides.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-10220317, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-1133335, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-13910965, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-13929834, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-1508986, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-1780364, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-1784644, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-2100524, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-2605243, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-2825124, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-2838824, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-2847910, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-3217442, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-3903794, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-4512334, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-4608848, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-4618356, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-4633460, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-4888976, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-4960673, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-5540307, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-5767305, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-5772779, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-6033613, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-6065103, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-7045692, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-7063608, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-7391221, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-8068635, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-8218287, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-866449, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-9146690, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-9240832, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-9301039, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-9358139, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-9461471, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-981339, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653818-9888851
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1042-58
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Sequence dependence of energy transfer in DNA oligonucleotides.
pubmed:affiliation
Department of Physics, University of Alabama at Birmingham, Birmingham, Alabama 35294-1170, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.