Linked Life Data

10653813

Source:http://linkedlifedata.com/resource/pubmed/id/10653813

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-2-25
pubmed:abstractText
Iron-induced flavocytochrome c(3), Ifc(3), from Shewanella frigidimarina NCIMB400, derivatized with a 2-pyridyl disulfide label, self-assembles on gold electrodes as a functional array whose fumarate reductase activity as viewed by direct electrochemistry is indistinguishable from that of Ifc(3) adsorbed on gold or graphite electrodes. The enhanced stability of the labeled protein's array permits analysis at a rotating electrode and limiting catalytic currents fit well to a Michaelis-Menten description of enzyme kinetics with K(M) = 56 +/- 20 microM, pH 7.5, comparable to that obtained in solution assays. At fumarate concentrations above 145 microM cyclic voltammetry shows the catalytic response to contain two features. The position and width of the lower potential component centered on -290 mV and corresponding to a one-electron wave implicates the oxidation state of the lowest potential heme of Ifc(3) as a defining feature in the mechanism of fumarate reduction at high turnover rates. We propose the operation of dual pathways for electron transfer to the active site of Ifc(3) with the lowest potential heme acting as an electron relay on one of these pathways.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-10066163, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-10079073, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-10093731, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-10455032, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-10581549, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-17847385, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-5029854, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-5138340, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-646998, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-708370, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-8639579, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653813-9095195
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1001-9
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Voltammetry of a flavocytochrome c(3): the lowest potential heme modulates fumarate reduction rates.
pubmed:affiliation
Schools, Centre for Metalloprotein Spectroscopy and Biology, University of East Anglia, Norwich NR4 7TJ, United Kingdom. j.butt@uea.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't