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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-2-25
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pubmed:abstractText |
A method is presented for rapidly extracting single-channel transition rate constants from patch-clamp recordings containing signals from several channels. The procedure is based on a simultaneous fit of the observed dwell-time distributions for all conductance levels, using a maximum likelihood approach. This algorithm allows estimation of single-channel rate constants in cases where more advanced methods may be impractical because of their extremely long computational time. A correction is included for the limited time resolution of the recording system, according to theory developed by Roux and Sauvé (Biophys. J. 48:149-158, 1985), by accounting for the impact of undetected transitions on the dwell-time distributions, and by introducing an improved practical implementation of a fixed dead time for the case of more than one channel. This feature allows application of the method to noisy data, after filtering. A computer program implementing the method is tested successfully on a variety of simulated multichannel current traces.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-1280835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-1663796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-22856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-2410047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-2419552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-2447968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-2539443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-2579685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-6111797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-6301575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-6311301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-6317854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-8189206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-8189207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-8189208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-8770203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-9107053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-9168029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-9450944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-9450945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-9450946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653791-9705979
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
785-99
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10653791-Algorithms,
pubmed-meshheading:10653791-Data Interpretation, Statistical,
pubmed-meshheading:10653791-Electric Conductivity,
pubmed-meshheading:10653791-Ion Channel Gating,
pubmed-meshheading:10653791-Kinetics,
pubmed-meshheading:10653791-Markov Chains,
pubmed-meshheading:10653791-Models, Theoretical,
pubmed-meshheading:10653791-Patch-Clamp Techniques,
pubmed-meshheading:10653791-Software
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pubmed:year |
2000
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pubmed:articleTitle |
Rapid kinetic analysis of multichannel records by a simultaneous fit to all dwell-time histograms.
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pubmed:affiliation |
Laboratory of Cardiac/Membrane Physiology, The Rockefeller University, 1230 York Avenue, Box 297, New York, New York 10021, USA. csanadl@rockvax.rockefeller.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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