Linked Life Data

10653719

Source:http://linkedlifedata.com/resource/pubmed/id/10653719

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-3-13
pubmed:abstractText
The yield coefficient (YC) of Pseudomonas sp. strain DP-4, a 2, 4-dichlorophenol (DCP)-degrading organism, was estimated from the number of CFU produced at the expense of 1 unit amount of DCP at low concentrations. At a low concentration of DCP, the YC can be overestimated in pure culture, because DP-4 assimilated not only DCP but also uncharacterized organic compounds contaminating a mineral salt medium. The concentration of these uncharacterized organic compounds was nutritionally equivalent to 0.7 microg of DCP-C ml(-1). A mixed culture with non-DCP-degrading organisms resulted in elimination of ca. 99.9% of the uncharacterized organic compounds, and then DP-4 assimilated only DCP as a substrate. In a mixed culture, DP-4 degraded an initial concentration of 0.1 to 10 microg of C ml of DCP(-1) and the number of CFU of DP-4 increased. In the mixed culture, DCP at an initial concentration of 0.07 microg of C ml(-1) was degraded. However, the number of CFU of DP-4 did not increase. DCP at an extremely low initial concentration of 0.01 microg of C ml(-1) was not degraded in mixed culture even by a high density, 10(5) CFU ml(-1), of DP-4. When glucose was added to this mixed culture to a final concentration of 1 microg of C ml(-1), the initial concentration of 0.01 microg of C ml of DCP(-1) was degraded. These results suggested that DP-4 required cosubstrates to degrade DCP at an extremely low initial concentration of 0.01 microg of C ml(-1). The YCs of DP-4 at the expense of DCP alone decreased discontinuously with the decrease of the initial concentration of DCP, i.e., 1.5, 0.19, or 0 CFU per pg of DCP-C when 0.7 to 10, 0.1 to 0.5, or 0.07 microg of C ml of DCP(-1) was degraded, respectively. In this study, we developed a new method to eliminate uncharacterized organic compounds, and we estimated the YC of DP-4 at the expense of DCP as a sole source of carbon.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-2353833, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-3579282, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-3890738, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-4083891, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-4091549, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-4557166, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-4999114, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-6125958, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-6742843, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-8580642, http://linkedlifedata.com/resource/pubmed/commentcorrection/10653719-8759311
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
566-70
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Estimation of the yield coefficient of Pseudomonas sp. strain DP-4 with a low substrate (2,4-dichlorophenol [DCP]) concentration in a mineral medium from which uncharacterized organic compounds were eliminated by a non-DCP-degrading organism.
pubmed:affiliation
Laboratory of Environmental Microbiology, Department of Environmental and Natural Resource Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. tarao@cc.tuat.ac.jp
pubmed:publicationType
Journal Article