Linked Life Data

10653480

Source:http://linkedlifedata.com/resource/pubmed/id/10653480

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-3-6
pubmed:abstractText
In the present study we have observed a higher state of coupling in respiring mitochondria isolated from green as compared to red tomatoes (Lycopersicon esculentum, Mill.). Green tomato mitochondria produced a membrane potential (deltapsi) high enough to phosphorylate ADP, whereas in red tomato mitochondria, BSA and ATP were required to restore deltapsi to the level of that obtained with green tomato mitochondria. This supports the notion that such uncoupling in red tomato mitochondria is mediated by a plant uncoupling mitochondrial protein (PUMP; cf. Vercesi et al., 1995). Nevertheless, mitochondria from both green and red tomatoes exhibited an ATP-sensitive linoleic acid (LA)-induced deltapsi decrease providing evidence that PUMP is also present in green tomatoes. Indeed, proteoliposomes containing reconstituted green or red tomato PUMP showed LA uniport and LA-induced H+ transport. It is suggested that the higher concentration of free fatty acids (PUMP substrates) in red tomatoes could explain the lower coupling state in mitochondria isolated from these fruits.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0145-479X
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
527-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Plant uncoupling mitochondrial protein activity in mitochondria isolated from tomatoes at different stages of ripening.
pubmed:affiliation
Departamento de Patologia Clínica (NMCE), Faculdade de Ciências Médicas, Universidade Estadual de Campinas, SP, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't