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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2000-3-2
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pubmed:abstractText |
The AML-1-encoded transcription factor, AML-1B, regulates numerous hematopoietic-specific genes. Inappropriate expression of AML-1-family proteins is oncogenic in cell culture systems and in mice. To understand the oncogenic functions of AML-1, we established cell lines expressing AML-1B to examine the role of AML-1 in the cell cycle. DNA content analysis and bromodeoxyuridine pulse-chase studies indicated that entry into the S phase of the cell cycle was accelerated by up to 4 h in AML-1B-expressing 32D.3 myeloid progenitor cells as compared with control cells or cells expressing E2F-1. However, AML-1B was not able to induce continued cell cycle progression in the absence of growth factors. The DNA binding and transactivation domains of AML-1B were required for altering the cell cycle. Thus, AML-1B is the first transcription factor that affects the timing of the mammalian cell cycle.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3438-45
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10652337-Animals,
pubmed-meshheading:10652337-Cell Cycle,
pubmed-meshheading:10652337-Cell Line,
pubmed-meshheading:10652337-Core Binding Factor Alpha 2 Subunit,
pubmed-meshheading:10652337-DNA-Binding Proteins,
pubmed-meshheading:10652337-Flow Cytometry,
pubmed-meshheading:10652337-G1 Phase,
pubmed-meshheading:10652337-Gene Expression Regulation,
pubmed-meshheading:10652337-Humans,
pubmed-meshheading:10652337-Mice,
pubmed-meshheading:10652337-Proto-Oncogene Proteins,
pubmed-meshheading:10652337-Transcription Factors,
pubmed-meshheading:10652337-Transfection
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pubmed:year |
2000
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pubmed:articleTitle |
Expression of the AML-1 oncogene shortens the G(1) phase of the cell cycle.
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pubmed:affiliation |
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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