Linked Life Data

10652257

Source:http://linkedlifedata.com/resource/pubmed/id/10652257

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2000-4-12
pubmed:abstractText
Fibroblast growth factor-mediated signalling was studied in porcine aortic endothelial cells expressing either wild-type fibroblast growth factor receptor-1 or a mutant receptor (Y766F) unable to bind phospholipase C-(&ggr;). Stimulation of cells expressing the wild-type receptor resulted in activation of phospholipases C, D and A(2) and increased phosphoinositide 3-kinase activity. Stimulation of the wild-type receptor also resulted in stress fibre formation and a cellular shape change. Cells expressing the Y766F mutant receptor failed to stimulate phospholipase C, D and A(2) as well as phosphoinositide 3-kinase. Furthermore, no stress fibre formation or shape change was observed. Both the wild-type and Y766F receptor mutant activated MAP kinase and elicited proliferative responses in the porcine aortic endothelial cells. Thus, fibroblast growth factor receptor-1 mediated activation of phospholipases C, D and A(2) and phosphoinositide 3-kinase was dependent on tyrosine 766. Furthermore, whilst tyrosine 766 was not required for a proliferative response, it was required for fibroblast growth factor receptor-1 mediated cytoskeletal reorganisation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
113 ( Pt 4)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
643-51
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10652257-Animals, pubmed-meshheading:10652257-Aorta, pubmed-meshheading:10652257-Cell Division, pubmed-meshheading:10652257-Cells, Cultured, pubmed-meshheading:10652257-Cytoskeleton, pubmed-meshheading:10652257-Endothelium, Vascular, pubmed-meshheading:10652257-Mitogen-Activated Protein Kinases, pubmed-meshheading:10652257-Mutagenesis, pubmed-meshheading:10652257-Phosphatidylinositol 3-Kinases, pubmed-meshheading:10652257-Phospholipase D, pubmed-meshheading:10652257-Phospholipases, pubmed-meshheading:10652257-Phospholipases A, pubmed-meshheading:10652257-Phosphorylation, pubmed-meshheading:10652257-Receptor, Fibroblast Growth Factor, Type 1, pubmed-meshheading:10652257-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:10652257-Receptors, Fibroblast Growth Factor, pubmed-meshheading:10652257-Signal Transduction, pubmed-meshheading:10652257-Swine, pubmed-meshheading:10652257-Transfection, pubmed-meshheading:10652257-Type C Phospholipases, pubmed-meshheading:10652257-Tyrosine
pubmed:year
2000
pubmed:articleTitle
Tyrosine 766 in the fibroblast growth factor receptor-1 is required for FGF-stimulation of phospholipase C, phospholipase D, phospholipase A(2), phosphoinositide 3-kinase and cytoskeletal reorganisation in porcine aortic endothelial cells.
pubmed:affiliation
Department of Genetics, Rudbeck Laboratory, S-75185 Uppsala, Sweden. michael.cross@genpat.uu.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't