Source:http://linkedlifedata.com/resource/pubmed/id/10650930
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2000-2-10
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| pubmed:abstractText |
GH promptly increases cytosolic free calcium ([Ca2+]i) in freshly isolated rat adipocytes. Adipocytes deprived of GH for 3 h or longer are incapable of increasing [Ca2+]i in response to GH, but instead respond in an insulin-like manner. Insulin blocks the GH-induced increase in [Ca2+]i in GH-replete cells and stimulates the sodium pump (i.e. Na+/K+-ATPase), thereby hyperpolarizing the cell membrane. Blockade of the Na+/K+-ATPase with 100 microM ouabain reversed these effects of insulin and enabled GH to increase [Ca2+]i in GH-deprived adipocytes. Both insulin and GH activated the sodium pump in GH-deprived adipocytes, as indicated by increased uptake of 86Rb+. Decreasing availability of intracellular Na+ by blockade of Na+/K+/ 2Cl- symporters or Na+/H+ antiporters abolished the effects of both hormones on 86Rb+ uptake and enabled both GH and insulin to increase [Ca2+]i in GH-deprived adipocytes. The data suggest that hormonal stimulation of Na+/K+-ATPase activity interferes with activation of voltage-sensitive calcium channels by either membrane hyperpolarization or some unknown interaction between the sodium pump and calcium channels.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bumetanide,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Nimodipine,
http://linkedlifedata.com/resource/pubmed/chemical/Ouabain,
http://linkedlifedata.com/resource/pubmed/chemical/Rubidium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0013-7227
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
141
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
513-9
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10650930-Adipocytes,
pubmed-meshheading:10650930-Animals,
pubmed-meshheading:10650930-Biological Transport,
pubmed-meshheading:10650930-Bumetanide,
pubmed-meshheading:10650930-Calcium,
pubmed-meshheading:10650930-Cells, Cultured,
pubmed-meshheading:10650930-Cytosol,
pubmed-meshheading:10650930-Enzyme Activation,
pubmed-meshheading:10650930-Growth Hormone,
pubmed-meshheading:10650930-Insulin,
pubmed-meshheading:10650930-Kinetics,
pubmed-meshheading:10650930-Male,
pubmed-meshheading:10650930-Models, Biological,
pubmed-meshheading:10650930-Nimodipine,
pubmed-meshheading:10650930-Ouabain,
pubmed-meshheading:10650930-Rats,
pubmed-meshheading:10650930-Rubidium,
pubmed-meshheading:10650930-Sodium-Potassium-Exchanging ATPase
|
| pubmed:year |
2000
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| pubmed:articleTitle |
Activation of the sodium pump blocks the growth hormone-induced increase in cytosolic free calcium in rat adipocytes.
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| pubmed:affiliation |
Department of Physiology, University of Massachusetts Medical School, Worcester 01655, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|