|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5453
|
|
pubmed:dateCreated |
2000-2-18
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The polyketide epothilone is a potential anticancer agent that stabilizes microtubules in a similar manner to Taxol. The gene cluster responsible for epothilone biosynthesis in the myxobacterium Sorangium cellulosum was cloned and completely sequenced. It encodes six multifunctional proteins composed of a loading module, one nonribosomal peptide synthetase module, eight polyketide synthase modules, and a P450 epoxidase that converts desoxyepothilone into epothilone. Concomitant expression of these genes in the actinomycete Streptomyces coelicolor produced epothilones A and B. Streptomyces coelicolor is more amenable to strain improvement and grows about 10-fold as rapidly as the natural producer, so this heterologous expression system portends a plentiful supply of this important agent.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Epothilones,
http://linkedlifedata.com/resource/pubmed/chemical/Epoxy Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/epothilone A,
http://linkedlifedata.com/resource/pubmed/chemical/epothilone B,
http://linkedlifedata.com/resource/pubmed/chemical/epoxidase
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0036-8075
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
28
|
|
pubmed:volume |
287
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
640-2
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:10649995-Bacterial Proteins,
pubmed-meshheading:10649995-Cloning, Molecular,
pubmed-meshheading:10649995-Cosmids,
pubmed-meshheading:10649995-Cytochrome P-450 Enzyme System,
pubmed-meshheading:10649995-Epothilones,
pubmed-meshheading:10649995-Epoxy Compounds,
pubmed-meshheading:10649995-Fermentation,
pubmed-meshheading:10649995-Genes, Bacterial,
pubmed-meshheading:10649995-Molecular Sequence Data,
pubmed-meshheading:10649995-Multienzyme Complexes,
pubmed-meshheading:10649995-Multigene Family,
pubmed-meshheading:10649995-Myxococcales,
pubmed-meshheading:10649995-Operon,
pubmed-meshheading:10649995-Oxidoreductases,
pubmed-meshheading:10649995-Recombinant Proteins,
pubmed-meshheading:10649995-Streptomyces,
pubmed-meshheading:10649995-Thiazoles,
pubmed-meshheading:10649995-Transformation, Genetic
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Cloning and heterologous expression of the epothilone gene cluster.
|
|
pubmed:affiliation |
KOSAN Biosciences, 3832 Bay Center Place, Hayward, CA 94545, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
