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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1976-9-2
|
| pubmed:abstractText |
Crystals of solid phase-derived semisynthetic ribonuclease-S' were prepared and compared with those for native ribonuclease-S' and -S. The semisynthetic species used was the noncovalent complex of synthetic fragment-(1-20), corresponding to residues 1 through 20 of bovine pancreatic ribonuclease-A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase, EC 3.1.4.22), and native ribonuclease-S-(21-124); the fragment containing residues 21 through 124 of ribonuclease-A. This semisynthetic complex was completely active enzymatically, was homogeneous as judged by polyacrylamide gel electrophoresis, and had no greater than trace amounts of excess ribonuclease-s(21-124) as judged by affinity chromatography. Crystallization of both semisynthetic and native ribonuclease-s' at pH 5.3 resulted in well-formed crystallseater than trace amounts of excess ribonuclease-S-T21-124) as judged by affinity chromatography. Crystallization of both semisynthetic and native ribonuclease-S' at pH 5.3 resulted in well-formed crystals with the symmetry of space group P3121 and unit cell dimensions a=b=44.82, c=97.3 A. This crystal form corresponds to the Y form of native ribonuclease-S previously reported [Wyckoff et al. (1967) J. Biol. Chem. 242, 3749-3753]. X-ray diffraction patterns of the crystals were indistinguishable, indicative of the structural identity of semisynthetic and native ribonuclease-S'.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-1152063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-13654398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-14491328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-4319111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-4324344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-4420810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-4684711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-5127932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-5261024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-5319951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-5460889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-5679174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-6030327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1064856-6038503
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0027-8424
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
73
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1844-7
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading | |
| pubmed:year |
1976
|
| pubmed:articleTitle |
Crystalline semisynthetic ribonuclease-S'.
|
| pubmed:publicationType |
Journal Article
|