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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-2-24
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pubmed:abstractText |
The ets-family transcription factor PU.1 is required for the proper development of both myeloid and lymphoid progenitors. We used PU. 1-deficient animals to examine the role of PU.1 during dendritic cell development. PU.1(-/-)animals produce lymphoid-derived dendritic cells (DC): low-density class II major histocompatibility complex [MHC-II(+)] CD11c(+) CD8alpha(+) DEC-205(+). But they lack myeloid-derived DC: low-density MHC-II(+) CD11c(+) CD8alpha(-) DEC-205(-). PU.1(-/-) embryos also lack progenitors capable of differentiating into myeloid DC in response to granulocyte-macrophage colony-stimulating factor plus interleukin-4. The appearance of lymphoid DC in developing PU.1(-/-)thymus was initially delayed, but this population recovered to wild type (WT) levels upon organ culture of isolated thymic lobes. PU. 1(-/-)lymphoid DC were functionally equivalent to WT DC for stimulating T-cell proliferation in mixed lymphocyte reactions. These results demonstrate that PU.1 is required for the development of myeloid DC but not lymphoid DC.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
879-85
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10648399-Animals,
pubmed-meshheading:10648399-Antigen Presentation,
pubmed-meshheading:10648399-Bone Marrow,
pubmed-meshheading:10648399-Bone Marrow Cells,
pubmed-meshheading:10648399-Cell Lineage,
pubmed-meshheading:10648399-Clonal Deletion,
pubmed-meshheading:10648399-Dendritic Cells,
pubmed-meshheading:10648399-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:10648399-Hematopoiesis,
pubmed-meshheading:10648399-Immunophenotyping,
pubmed-meshheading:10648399-Interleukin-4,
pubmed-meshheading:10648399-Lymphoid Tissue,
pubmed-meshheading:10648399-Mice,
pubmed-meshheading:10648399-Mice, Knockout,
pubmed-meshheading:10648399-Organ Culture Techniques,
pubmed-meshheading:10648399-Proto-Oncogene Proteins,
pubmed-meshheading:10648399-Thymus Gland,
pubmed-meshheading:10648399-Trans-Activators
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pubmed:year |
2000
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pubmed:articleTitle |
PU.1 is required for myeloid-derived but not lymphoid-derived dendritic cells.
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pubmed:affiliation |
Institute for Human Gene Therapy, University of Pennsylvania, Philadelphia, PA 19104-6140, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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