Source:http://linkedlifedata.com/resource/pubmed/id/10647887
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2000-2-16
|
| pubmed:abstractText |
Among fifteen male skin fibroblast cultures from eleven donors ranging in age from less than 1 year to 90 years old, the specific activity of monoamine oxidase A (MAO-A) differed 515-fold. Each culture had one of the two most common alleles (three or four 30-bp repeats) at the variable number tandem repeat locus positioned 1.2 kb upstream from MAOA exon 1 (uVNTR). The mean MAO-A activity in cultures with three uVNTR repeats was significantly lower than that in cultures with four repeats (1.6 +/- 1.1 and 13 +/- 12 nmol/h per milligram, respectively; P=0.032). MAO-A expression was confined to a cell sub-population varying from 0.5% to 90% of cells in different cultures. The mean specific activity in MAO-A+ cells (whole culture specific activity divided by the proportion of immunopositive cells) was lower for cultures with three repeats than for those with four (7.2 +/- 3.1 and 23.9 +/- 9.5 nmol/h per milligram protein, respectively; P=0.0013), with no overlap in activity between genotypes. Finding lower MAO-A activity in cultures with three uVNTR repeats compared to those with four is consistent with published evidence that MAO-A promoter constructs bearing three repeats have lower transcriptional activity in transfected neuroblastoma and choriocarcinoma cells. The uVNTR genotype may be a common genetic determinant of significant individual differences in oxidizing capacity for critical MAO-A substrates, which include serotonin, norepinephrine, and tyramine.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0340-6717
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
105
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
542-51
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10647887-Adolescent,
pubmed-meshheading:10647887-Adult,
pubmed-meshheading:10647887-Aged,
pubmed-meshheading:10647887-Aged, 80 and over,
pubmed-meshheading:10647887-Cells, Cultured,
pubmed-meshheading:10647887-Child,
pubmed-meshheading:10647887-Child, Preschool,
pubmed-meshheading:10647887-Fibroblasts,
pubmed-meshheading:10647887-Flow Cytometry,
pubmed-meshheading:10647887-Fluorescent Antibody Technique,
pubmed-meshheading:10647887-Genotype,
pubmed-meshheading:10647887-Humans,
pubmed-meshheading:10647887-Infant,
pubmed-meshheading:10647887-Infant, Newborn,
pubmed-meshheading:10647887-Male,
pubmed-meshheading:10647887-Middle Aged,
pubmed-meshheading:10647887-Minisatellite Repeats,
pubmed-meshheading:10647887-Monoamine Oxidase,
pubmed-meshheading:10647887-Promoter Regions, Genetic,
pubmed-meshheading:10647887-Skin
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Association between monoamine oxidase A activity in human male skin fibroblasts and genotype of the MAOA promoter-associated variable number tandem repeat.
|
| pubmed:affiliation |
Department of Human Biological Chemistry and Genetics, Graduate School of Biomedical Sciences, University of Texas Medical Branch, Galveston 77555-0650, USA. rdenney@utmb.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|