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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2000-2-3
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pubmed:databankReference |
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pubmed:abstractText |
Lactose repressor protein (Lac) controls the expression of the lactose metabolic genes in Escherichia coli by binding to an operator sequence in the promoter of the lac operon. Binding of inducer molecules to the Lac core domain induces changes in tertiary structure that are propagated to the DNA-binding domain through the connecting hinge region, thereby reducing the affinity for the operator. Protein-protein and protein-DNA interactions involving the hinge region play a crucial role in the allosteric changes occurring upon induction, but have not, as yet, been analyzed in atomic detail.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0969-2126
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1483-92
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10647179-Bacterial Proteins,
pubmed-meshheading:10647179-Base Sequence,
pubmed-meshheading:10647179-Binding Sites,
pubmed-meshheading:10647179-Crystallography, X-Ray,
pubmed-meshheading:10647179-DNA, Bacterial,
pubmed-meshheading:10647179-Dimerization,
pubmed-meshheading:10647179-Escherichia coli,
pubmed-meshheading:10647179-Escherichia coli Proteins,
pubmed-meshheading:10647179-Hydrogen Bonding,
pubmed-meshheading:10647179-Lac Operon,
pubmed-meshheading:10647179-Lac Repressors,
pubmed-meshheading:10647179-Molecular Sequence Data,
pubmed-meshheading:10647179-Nuclear Magnetic Resonance, Biomolecular,
pubmed-meshheading:10647179-Nucleic Acid Conformation,
pubmed-meshheading:10647179-Oligodeoxyribonucleotides,
pubmed-meshheading:10647179-Promoter Regions, Genetic,
pubmed-meshheading:10647179-Protein Conformation,
pubmed-meshheading:10647179-Protein Structure, Secondary,
pubmed-meshheading:10647179-Repressor Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator.
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pubmed:affiliation |
Bijvoet Center for Biomolecular Research, Utrecht University, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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