Source:http://linkedlifedata.com/resource/pubmed/id/10646973
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2000-2-8
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pubmed:databankReference | |
pubmed:abstractText |
Xanthotoxin, a plant allelochemical, induces alpha-cypermethrin insecticide tolerance in Helicoverpa zea (corn earworm); inhibition of tolerance by piperonyl butoxide implicates cytochrome P450 monooxygenases (P450s) in the detoxification of this insecticide. To characterize the xanthotoxin-inducible P450 that might mediate alpha-cypermethrin tolerance in this species, a cDNA library prepared from xanthotoxin-induced H. zea fifth instar larvae was screened with cDNAs encoding furanocoumarin-metabolizing P450s from Papilio polyxenes (CYP6B1v2) and P. glaucus (CYP6B4v2) as well as a sequence-related P450 from Helicoverpa armigera (CYP6B2). One full-length cDNA isolated in this screening shares 51-99% amino acid identity with the CYP6B subfamily of P450s isolated from Papilio and Helicoverpa species and, thus, has been designated CYP6B8. All of these CYP6B subfamily members share a number of highly conserved domains, including substrate recognition site 1 (SRS 1) that is critical for xanthotoxin metabolism by CYP6B1v2 from Papilio polyxenes and coumarin metabolism by CYP2a5 from Mus musculus. Northern and RT-PCR analyses indicate that CYP6B8 expression is strongly induced by xanthotoxin and phenobarbital and negligibly induced by alpha-cypermethrin.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Insecticides,
http://linkedlifedata.com/resource/pubmed/chemical/Methoxsalen,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrethrins,
http://linkedlifedata.com/resource/pubmed/chemical/cypermethrin
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0965-1748
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
75-84
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10646973-Amino Acid Sequence,
pubmed-meshheading:10646973-Animals,
pubmed-meshheading:10646973-Base Sequence,
pubmed-meshheading:10646973-Cytochrome P-450 Enzyme System,
pubmed-meshheading:10646973-DNA, Complementary,
pubmed-meshheading:10646973-Insecticide Resistance,
pubmed-meshheading:10646973-Insecticides,
pubmed-meshheading:10646973-Lepidoptera,
pubmed-meshheading:10646973-Methoxsalen,
pubmed-meshheading:10646973-Molecular Sequence Data,
pubmed-meshheading:10646973-Pyrethrins,
pubmed-meshheading:10646973-Sequence Analysis, DNA
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pubmed:year |
2000
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pubmed:articleTitle |
Molecular cloning and expression of CYP6B8: a xanthotoxin-inducible cytochrome P450 cDNA from Helicoverpa zea.
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pubmed:affiliation |
Department of Plant Protection, Nanjing Agricultural University, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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