Source:http://linkedlifedata.com/resource/pubmed/id/10645895
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2000-3-31
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pubmed:abstractText |
We previously demonstrated that bovine serum conglutinin has markedly greater ability to inhibit influenza A virus (IAV) infectivity than other collectins. We now show that recombinant conglutinin and a chimeric protein containing the NH(2) terminus and collagen domain of rat pulmonary surfactant protein D (rSP-D) fused to the neck region and carbohydrate recognition domain (CRD) of conglutinin (termed SP-D/Cong(neck+CRD)) have markedly greater ability to inhibit infectivity of IAV than wild-type recombinant rSP-D, confirming that the potent IAV-neutralizing activity of conglutinin resides in its neck region and CRD. Furthermore, by virtue of incorporation of the NH(2) terminus and collagen domain of SP-D, SP-D/Cong(neck+CRD) caused substantially greater aggregation of IAV particles and enhancement of neutrophil binding of, and H(2)O(2) responses to, IAV than recombinant conglutinin or recombinant rSP-D. Hence, SP-D/Cong(neck+CRD) combined favorable antiviral and opsonic properties of conglutinin and SP-D. This study demonstrates an association of specific structural domains of SP-D and conglutinin with specific functional properties and illustrates that antimicrobial activities of wild-type collectins can be enhanced through recombinant strategies.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collectins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Globulins,
http://linkedlifedata.com/resource/pubmed/chemical/conglutinin
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1040-0605
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
L90-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10645895-Animals,
pubmed-meshheading:10645895-Cattle,
pubmed-meshheading:10645895-Chimera,
pubmed-meshheading:10645895-Collectins,
pubmed-meshheading:10645895-Glycoproteins,
pubmed-meshheading:10645895-Hemagglutination,
pubmed-meshheading:10645895-Humans,
pubmed-meshheading:10645895-Hydrogen Peroxide,
pubmed-meshheading:10645895-Influenza, Human,
pubmed-meshheading:10645895-Influenza A virus,
pubmed-meshheading:10645895-Neutrophils,
pubmed-meshheading:10645895-Peptide Fragments,
pubmed-meshheading:10645895-Pulmonary Surfactant-Associated Protein D,
pubmed-meshheading:10645895-Pulmonary Surfactants,
pubmed-meshheading:10645895-Rats,
pubmed-meshheading:10645895-Recombinant Fusion Proteins,
pubmed-meshheading:10645895-Serum Globulins,
pubmed-meshheading:10645895-Virion
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pubmed:year |
2000
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pubmed:articleTitle |
Enhanced anti-influenza activity of a surfactant protein D and serum conglutinin fusion protein.
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pubmed:affiliation |
Departments of Medicine and Pathology, Boston University School of Medicine, Boston, Massachusetts 02118, USA. khartsho@bu.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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