Source:http://linkedlifedata.com/resource/pubmed/id/10644965
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2000-3-2
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pubmed:abstractText |
Growth stimulation of periimplant tissues by growth factors like transforming growth factor-beta1 (TGF-beta1) may increase the indication for and success of implant use. Calcium phosphate as a material for implants or for coating of implants is known for its good biologic interaction with bone. Therefore, calcium phosphate implants combined with TGF-beta1 might improve osseointegration. In this study we hypothesise that the addition of recombinant human TGF-beta1 (rhTGF-beta1) to calcium phosphate cement (CPC) affects the differentiation of bone cells growing on the cement layer. rhTGF-beta1 incorporated during setting in a CPC layer at 20 ng rhTGF-beta1/60 mg cement was found to be gradually released into tissue culturing medium leading to a 20% release after 24 h. Two cell populations were obtained from collagenase-treated fragments of adult rat long bones: preosteoblastic cells, which were released by the collagenase treatment, and osteoblastic cells, which grew from the collagenase-stripped bone fragments. Both cell populations were tested for their osteoblastic characteristic phenotype by measuring their alkaline phosphatase (ALP) activity after vitamin D treatment and cyclic AMP after parathyroid hormone stimulation. After preculture the cells were plated on a layer of CPC containing 0 (control), 10, or 20 ng rhTGF-beta1/60 mg CPC. Bone cell differentiation was analyzed after 10 days by measuring the ALP activity, as well as the protein content of the cell layer. Incorporation of rhTGF-beta1 in the CPC did not change the ALP activity in osteoblastic cells, but a significant (analyzed by multivariate analysis of variance) increase was observed in preosteoblastic cells. Incorporation of 10 ng of rhTGF-beta1 in 60 mg of CPC increased the ALP activity in preosteoblastic cells by threefold and 20 ng rhTGF-beta1/60 mg CPC increased it by fivefold. The total protein content was not affected by rhTGF-beta1 in either of the cell populations. We conclude that rhTGF-beta1 incorporated during setting in CPC stimulates the differentiation of preosteoblastic cells in vitro. These results provide a basis for further studies on the use of this combination as an implant material in vivo.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-3-isobutylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Cements,
http://linkedlifedata.com/resource/pubmed/chemical/Calcitriol,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Parathyroid Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9304
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2000 John Wiley & Sons, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
67-74
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10644965-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:10644965-Alkaline Phosphatase,
pubmed-meshheading:10644965-Animals,
pubmed-meshheading:10644965-Biological Markers,
pubmed-meshheading:10644965-Bone Cements,
pubmed-meshheading:10644965-Bone Regeneration,
pubmed-meshheading:10644965-Bone and Bones,
pubmed-meshheading:10644965-Calcitriol,
pubmed-meshheading:10644965-Calcium Phosphates,
pubmed-meshheading:10644965-Cell Differentiation,
pubmed-meshheading:10644965-Cells, Cultured,
pubmed-meshheading:10644965-Humans,
pubmed-meshheading:10644965-Osteoblasts,
pubmed-meshheading:10644965-Parathyroid Hormone,
pubmed-meshheading:10644965-Rats,
pubmed-meshheading:10644965-Recombinant Proteins,
pubmed-meshheading:10644965-Transforming Growth Factor beta
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pubmed:year |
2000
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pubmed:articleTitle |
Transforming growth factor-beta1 incorporated during setting in calcium phosphate cement stimulates bone cell differentiation in vitro.
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pubmed:affiliation |
Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam-Vrije Universiteit, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands.e.blom.ocb.acta@med.vu.nl
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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