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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-3-31
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pubmed:abstractText |
The renal urea transporter gene (UT-A) produces different transcripts in the inner medullary collecting ducts (UT-A1) and thin descending limbs of Henle's loop (UT-A2), coding for distinct proteins. Peptide-directed rabbit polyclonal antibodies were used to identify the UT-A2 protein in renal medulla of mouse and rat. In the inner stripe of outer medulla, an antibody directed to the COOH terminus of UT-A recognized a membrane protein of 55 kDa. The abundance of this 55-kDa protein was strongly increased in response to chronic infusion of the vasopressin analog 1-deamino-[8-D-arginine]vasopressin (DDAVP) in Brattleboro rats, consistent with previous evidence that UT-A2 mRNA abundance is markedly increased. Immunofluorescence labeling with the COOH-terminal antibody in Brattleboro rats revealed labeling in the lower portion of descending limbs from short-looped nephrons (in the aquaporin-1-negative portion of this segment). This UT-A labeling was increased in response to DDAVP. Increased labeling was also seen in descending limbs of long-looped nephrons in the base of the inner medulla. These results indicate that UT-A2 is expressed as a 55-kDa protein in portions of the thin descending limbs of Henle's loop and that the abundance of this protein is strongly upregulated by vasopressin.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Aqp1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Aqp1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Aquaporin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Aquaporins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deamino Arginine Vasopressin,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Vasopressins,
http://linkedlifedata.com/resource/pubmed/chemical/urea transporter
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1931-857X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
F52-62
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:10644655-Animals,
pubmed-meshheading:10644655-Antibodies,
pubmed-meshheading:10644655-Aquaporin 1,
pubmed-meshheading:10644655-Aquaporins,
pubmed-meshheading:10644655-Carrier Proteins,
pubmed-meshheading:10644655-Deamino Arginine Vasopressin,
pubmed-meshheading:10644655-Fluorescent Antibody Technique,
pubmed-meshheading:10644655-Immunoblotting,
pubmed-meshheading:10644655-Kidney Tubules, Proximal,
pubmed-meshheading:10644655-Male,
pubmed-meshheading:10644655-Membrane Glycoproteins,
pubmed-meshheading:10644655-Membrane Transport Proteins,
pubmed-meshheading:10644655-Mice,
pubmed-meshheading:10644655-Mice, Inbred BALB C,
pubmed-meshheading:10644655-Protein Isoforms,
pubmed-meshheading:10644655-Rats,
pubmed-meshheading:10644655-Rats, Sprague-Dawley,
pubmed-meshheading:10644655-Up-Regulation,
pubmed-meshheading:10644655-Vasopressins
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pubmed:year |
2000
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pubmed:articleTitle |
UT-A2: a 55-kDa urea transporter in thin descending limb whose abundance is regulated by vasopressin.
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pubmed:affiliation |
Department of Physiology, Univ. of Maryland School of Medicine, Baltimore, MD 21201, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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