| pubmed-article:10642295 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10642295 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:10642295 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:10642295 | lifeskim:mentions | umls-concept:C0008662 | lld:lifeskim |
| pubmed-article:10642295 | lifeskim:mentions | umls-concept:C0597336 | lld:lifeskim |
| pubmed-article:10642295 | lifeskim:mentions | umls-concept:C0005823 | lld:lifeskim |
| pubmed-article:10642295 | lifeskim:mentions | umls-concept:C0679199 | lld:lifeskim |
| pubmed-article:10642295 | lifeskim:mentions | umls-concept:C0678536 | lld:lifeskim |
| pubmed-article:10642295 | pubmed:issue | 1 Pt 2 | lld:pubmed |
| pubmed-article:10642295 | pubmed:dateCreated | 2000-2-7 | lld:pubmed |
| pubmed-article:10642295 | pubmed:abstractText | The identification of any quantitative trait locus (QTL) via a genome scan is only the first step toward the ultimate goal of gene identification. The next step is the production of congenic strains by which the existence of a QTL may be verified and the implicated chromosomal region be reduced to a size applicable to positional cloning of the causal gene. We used a speed congenic breeding protocol previously verified in mice for 2 blood pressure QTLs on rat chromosome 2. Four congenic strains were produced through introgression of various segments of chromosome 2 from Wistar-Kyoto rats from Glasgow colonies [WKY((Gla)) rats] into the recipient stroke-prone spontaneously hypertensive rats from Glasgow colonies [SHRSP((Gla))], and vice versa. The number of backcross generations required for each strain to achieve complete homozygosity at 83 background genetic markers in a "best" male varied between 3 and 4. Transfer of the region of rat chromosome 2 containing both QTLs from WKY((Gla)) into an SHRSP((Gla)) genetic background lowered both baseline and salt-loaded systolic blood pressure by approximately 20 and approximately 40 mm Hg in male congenic rats compared with the SHRSP parental strain (F=53.4, P<0.005; F=28.0, P< 0.0005, respectively). In contrast, control animals for stowaway heterozygosity presented no deviation from the blood pressure values recorded for the SHRSP((Gla)), indicating that if such heterozygosity exists, its effect on blood pressure is negligible. A reciprocal strategy in which 1 or both QTLs on rat chromosome 2 were transferred from SHRSP((Gla)) into a WKY((Gla)) genetic background resulted in statistically significant but smaller blood pressure increases for 1 of these QTLs. These results confirm the existence of blood pressure QTLs on rat chromosome 2 and demonstrate the applicability of a speed congenic strategy in the rat and emphasize the important role of the genetic background. | lld:pubmed |
| pubmed-article:10642295 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10642295 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10642295 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10642295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10642295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10642295 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10642295 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:10642295 | pubmed:issn | 0194-911X | lld:pubmed |
| pubmed-article:10642295 | pubmed:author | pubmed-author:GrahamDD | lld:pubmed |
| pubmed-article:10642295 | pubmed:author | pubmed-author:ClarkJ SJS | lld:pubmed |
| pubmed-article:10642295 | pubmed:author | pubmed-author:AndersonN HNH | lld:pubmed |
| pubmed-article:10642295 | pubmed:author | pubmed-author:GauguierDD | lld:pubmed |
| pubmed-article:10642295 | pubmed:author | pubmed-author:DominiczakA... | lld:pubmed |
| pubmed-article:10642295 | pubmed:author | pubmed-author:JeffsBB | lld:pubmed |
| pubmed-article:10642295 | pubmed:author | pubmed-author:NegrinC DCD | lld:pubmed |
| pubmed-article:10642295 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10642295 | pubmed:volume | 35 | lld:pubmed |
| pubmed-article:10642295 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10642295 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10642295 | pubmed:pagination | 179-87 | lld:pubmed |
| pubmed-article:10642295 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:10642295 | pubmed:meshHeading | pubmed-meshheading:10642295... | lld:pubmed |
| pubmed-article:10642295 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10642295 | pubmed:articleTitle | Applicability of a "speed" congenic strategy to dissect blood pressure quantitative trait loci on rat chromosome 2. | lld:pubmed |
| pubmed-article:10642295 | pubmed:affiliation | Department of Medicine and Therapeutics, University of Glasgow, Western Infirmary, Glasgow, UK. anna.dominiczak@clinmed.gla.ac.uk | lld:pubmed |
| pubmed-article:10642295 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10642295 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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