Source:http://linkedlifedata.com/resource/pubmed/id/10642295
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1 Pt 2
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pubmed:dateCreated |
2000-2-7
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pubmed:abstractText |
The identification of any quantitative trait locus (QTL) via a genome scan is only the first step toward the ultimate goal of gene identification. The next step is the production of congenic strains by which the existence of a QTL may be verified and the implicated chromosomal region be reduced to a size applicable to positional cloning of the causal gene. We used a speed congenic breeding protocol previously verified in mice for 2 blood pressure QTLs on rat chromosome 2. Four congenic strains were produced through introgression of various segments of chromosome 2 from Wistar-Kyoto rats from Glasgow colonies [WKY((Gla)) rats] into the recipient stroke-prone spontaneously hypertensive rats from Glasgow colonies [SHRSP((Gla))], and vice versa. The number of backcross generations required for each strain to achieve complete homozygosity at 83 background genetic markers in a "best" male varied between 3 and 4. Transfer of the region of rat chromosome 2 containing both QTLs from WKY((Gla)) into an SHRSP((Gla)) genetic background lowered both baseline and salt-loaded systolic blood pressure by approximately 20 and approximately 40 mm Hg in male congenic rats compared with the SHRSP parental strain (F=53.4, P<0.005; F=28.0, P< 0.0005, respectively). In contrast, control animals for stowaway heterozygosity presented no deviation from the blood pressure values recorded for the SHRSP((Gla)), indicating that if such heterozygosity exists, its effect on blood pressure is negligible. A reciprocal strategy in which 1 or both QTLs on rat chromosome 2 were transferred from SHRSP((Gla)) into a WKY((Gla)) genetic background resulted in statistically significant but smaller blood pressure increases for 1 of these QTLs. These results confirm the existence of blood pressure QTLs on rat chromosome 2 and demonstrate the applicability of a speed congenic strategy in the rat and emphasize the important role of the genetic background.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0194-911X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
179-87
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10642295-Animals,
pubmed-meshheading:10642295-Blood Pressure,
pubmed-meshheading:10642295-Chromosome Mapping,
pubmed-meshheading:10642295-Circadian Rhythm,
pubmed-meshheading:10642295-DNA, Satellite,
pubmed-meshheading:10642295-Female,
pubmed-meshheading:10642295-Genetic Markers,
pubmed-meshheading:10642295-Genotype,
pubmed-meshheading:10642295-Homozygote,
pubmed-meshheading:10642295-Hypertension,
pubmed-meshheading:10642295-Male,
pubmed-meshheading:10642295-Quantitative Trait, Heritable,
pubmed-meshheading:10642295-Rats,
pubmed-meshheading:10642295-Rats, Inbred SHR,
pubmed-meshheading:10642295-Rats, Inbred WKY,
pubmed-meshheading:10642295-Species Specificity,
pubmed-meshheading:10642295-Stroke
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pubmed:year |
2000
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pubmed:articleTitle |
Applicability of a "speed" congenic strategy to dissect blood pressure quantitative trait loci on rat chromosome 2.
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pubmed:affiliation |
Department of Medicine and Therapeutics, University of Glasgow, Western Infirmary, Glasgow, UK. anna.dominiczak@clinmed.gla.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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