Source:http://linkedlifedata.com/resource/pubmed/id/10640749
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2000-2-24
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| pubmed:abstractText |
There is evidence that donor-derived dendritic cells (DC), particularly those at a precursor/immature stage, may play a role in the immune privilege of liver allografts. Underlying mechanisms are poorly understood. We have examined the influence of in vitro generated mouse liver-derived DC progenitors (DCp) on proliferative, cytotoxic, and Th1/Th2 cytokine responses induced in allogeneic T cells. Liver DCp, propagated in GM-CSF from C57B10 mice (H2b), induced only minimal proliferation, and weak cytotoxic responses in allogeneic (C3H; H2k) T cells compared with mature bone marrow (BM)-derived DC. Flow-cytometric analysis of intracellular cytokine staining revealed that mature BM DC, but not liver DCp, elicited CD4+ T cell production of IFN-gamma. Intracellular expression of IL-10 was very low in both BM DC- and liver DCp-stimulated CD4+ T cells. Only stimulation by liver DCp was associated with IL-10 secretion in primary MLR. Notably, these liver DCp cocultured with allogeneic T cells stained strongly for IL-10. Following local (s.c. ) injection in allogeneic recipients, both BM DC and liver DCp homed to T cell areas of draining lymph nodes and spleen, where they were readily detected by immunohistochemistry up to 2 wk postinjection. Liver DCp induced clusters of IL-10- and IL-4-secreting mononuclear cells, whereas Th2 cytokine-secreting cells were not detected in mice injected with mature BM DC. By contrast, comparatively high numbers of IFN-gamma+ cells were induced by BM DC. Modulation of Th2 cytokine production by donor-derived DCp may contribute to the comparative immune privilege of hepatic allografts.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
164
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1346-54
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10640749-Animals,
pubmed-meshheading:10640749-Bone Marrow Cells,
pubmed-meshheading:10640749-Bone Marrow Transplantation,
pubmed-meshheading:10640749-Cells, Cultured,
pubmed-meshheading:10640749-Coculture Techniques,
pubmed-meshheading:10640749-Cytokines,
pubmed-meshheading:10640749-Cytotoxicity, Immunologic,
pubmed-meshheading:10640749-Dendritic Cells,
pubmed-meshheading:10640749-Immunophenotyping,
pubmed-meshheading:10640749-Interferon-gamma,
pubmed-meshheading:10640749-Interleukin-10,
pubmed-meshheading:10640749-Interleukin-4,
pubmed-meshheading:10640749-Intracellular Fluid,
pubmed-meshheading:10640749-Liver,
pubmed-meshheading:10640749-Liver Transplantation,
pubmed-meshheading:10640749-Lymphocyte Activation,
pubmed-meshheading:10640749-Mice,
pubmed-meshheading:10640749-Mice, Inbred C3H,
pubmed-meshheading:10640749-Mice, Inbred C57BL,
pubmed-meshheading:10640749-Stem Cell Transplantation,
pubmed-meshheading:10640749-Stem Cells,
pubmed-meshheading:10640749-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:10640749-Th2 Cells
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| pubmed:year |
2000
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| pubmed:articleTitle |
Effects of liver-derived dendritic cell progenitors on Th1- and Th2-like cytokine responses in vitro and in vivo.
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| pubmed:affiliation |
Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|