Linked Life Data

10640699

Source:http://linkedlifedata.com/resource/pubmed/id/10640699

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-4-27
pubmed:abstractText
cripto is the original member of the family of EGF-CFC genes, recently recognized as novel extracellular factors essential for vertebrate development. During the early stages of mouse gastrulation, cripto mRNA is detected in mesodermal cells; later, cripto mRNA is detected only in the truncus arteriosus of the developing heart. Here we describe the in vivo distribution of Cripto protein throughout mouse embryo development and show that cripto mRNA and protein colocalize. By means of immunofluorescence analysis and biochemical characterization, we show that Cripto is a membrane-bound protein anchored to the lipid bilayer by a glycosylphosphatidylinositol (GPI) moiety. We suggest that presentation of Cripto on the cell surface via a GPI-linkage is important in determining the spatial specificity of cell-cell interactions that play a critical role in the early patterning of the embryo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0925-4773
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-42
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:10640699-Animals, pubmed-meshheading:10640699-Binding Sites, pubmed-meshheading:10640699-Cell Line, Transformed, pubmed-meshheading:10640699-Cell Membrane, pubmed-meshheading:10640699-Embryonic and Fetal Development, pubmed-meshheading:10640699-Epidermal Growth Factor, pubmed-meshheading:10640699-GPI-Linked Proteins, pubmed-meshheading:10640699-Glycosylphosphatidylinositols, pubmed-meshheading:10640699-Humans, pubmed-meshheading:10640699-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:10640699-Membrane Glycoproteins, pubmed-meshheading:10640699-Mice, pubmed-meshheading:10640699-Neoplasm Proteins, pubmed-meshheading:10640699-Phosphatidylinositol Diacylglycerol-Lyase, pubmed-meshheading:10640699-Rabbits, pubmed-meshheading:10640699-Tumor Cells, Cultured, pubmed-meshheading:10640699-Type C Phospholipases
pubmed:year
2000
pubmed:articleTitle
Membrane-anchorage of Cripto protein by glycosylphosphatidylinositol and its distribution during early mouse development.
pubmed:affiliation
International Institute of Genetics and Biophysics, CNR, Via G. Marconi 12, 80125, Naples, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't