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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-2-10
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pubmed:abstractText |
Humans infected with the dimorphic fungus Blastomyces dermatitidis develop strong T-lymphocyte responses to WI-1, an immunodominant antigen that has been shown to elicit protective immunity in mice. In the present study, the T-cell epitopes of WI-1 and human leukocyte antigen (HLA) restricting elements that display them were investigated. Peripheral blood mononuclear cells (PBMC) from 37 patients with a confirmed history of blastomycosis were tested for a response to WI-1 in primary proliferation assays; PBMC from 35 (95%) responded. Six patients whose PBMC proliferated strongly in response to WI-1 (defined as a stimulation index greater than 50) were tested further for responses to subcloned, recombinant fragments of the antigen. These patients responded chiefly to sequences within the N terminus and the 25-amino-acid tandem repeat. Cloned CD4(+) T cells from an infected individual were used to delineate more precisely the peptide epitopes in the fragments and HLA restricting elements that present them. A majority of the T-cell clones recognized an epitope spanning amino acids 149 to 172 within the N terminus, displayed by HLA-DR 15. A minority of the clones, which have been shown to perform a cytolytic function in vitro, recognized an epitope in the tandem repeat displayed by HLA-DPw4, an uncommon restricting element. Tandem repeat epitopes required display by the beta chain of DPw4 heterodimers. Thus, human T cells with different functions in vitro also recognize distinct regions of WI-1, raising the possibility that HLA restricting elements that present them could modulate immunity during blastomycosis by selection and display of WI-1 peptides.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-10209038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-1383148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-1567099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-1833816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-2108045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-2295693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-3171821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-3945290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-6192342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-6417068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-6966655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-7399670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-7529285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-8326001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-8412644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-8530512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-8636413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-8843223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-8906834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-9057353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-9336663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-9367819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-9577091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10639410-9784555
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
502-10
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10639410-Amino Acid Sequence,
pubmed-meshheading:10639410-Antigens, Fungal,
pubmed-meshheading:10639410-Blastomyces,
pubmed-meshheading:10639410-Blastomycosis,
pubmed-meshheading:10639410-Epitopes, T-Lymphocyte,
pubmed-meshheading:10639410-HLA Antigens,
pubmed-meshheading:10639410-HLA-DP Antigens,
pubmed-meshheading:10639410-HLA-DR Antigens,
pubmed-meshheading:10639410-Humans,
pubmed-meshheading:10639410-Molecular Sequence Data
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pubmed:year |
2000
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pubmed:articleTitle |
T-Cell epitopes and human leukocyte antigen restriction elements of an immunodominant antigen of Blastomyces dermatitidis.
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pubmed:affiliation |
Department of Internal Medicine, Louisiana State University Medical School, Shreveport, Louisiana 71103, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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