Source:http://linkedlifedata.com/resource/pubmed/id/10639010
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2000-2-11
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| pubmed:abstractText |
In cultured endothelial cells harvested from human umbilical vein (HUVEC) or bovine aorta (BAEC) the 30 min incubation with calcium ionophore A 23187 (1 microM) or ticlopidine (100 microM) caused an increase in nitrite generation in HUVEC from basal 227 +/- 37 to 372 +/- 60 or to 325 +/- 33 pmoles per 10(6) cells, respectively, and in BAEC from basal 182 +/- 17 to 378 +/- 18 or to 423 +/- 66 pmoles per 106 cells (n = 6), respectively. Calcium ionophore A 23187 (1 microM) or ticlopidine (100 microM) next to 30 min incubation with BAEC increased release of 6-keto-PGF 1alpha from basal level of 9.4 +/- 1.8 to 96.2 +/- 5.1 or to 99.5 +/- 10.2 pmoles per 10(6) cells, respectively. The pretreatment with aspirin (300 microM) cut down this rise to 4.2 +/- 0.1 pmoles per 10(6) cells (n = 8). Basal cytoplasmic calcium levels, [Ca2+]i, in immortalised HUVEC cell line - ECV304, HUVEC and BAEC were 47.7 +/- 3.3 nM (n = 53), 68.3 +/- 5.0 nM (n = 30) and 53.1 +/- 3.0 nM (n = 15), respectively. In these cultured endothelial cells calcium ionophore A 23187 (0.1 microM) produced net maximum rise in [Ca2+]i by 157 +/-27 nM (n = 16)[ ECV304], by 107 +/- 58 nM (n=4) [HUVEC], and by 231.0 +/- 41.3 nM (n = 8) [BAEC], respectively, while ticlopidine (30 microM) produced net maximum rise in [Ca2+]i by 30.0 +/- 3.2 nM (n=9)[ECV304], 48.8 +/- 15.6 nM (n = 4)[HUVEC] and 28.4 +/- 5.4 nM (n = 8)[BAEC], respectively. Effect of ticlopidine on [Ca2+]i was not only weaker than that of calcium A 23187 but also its maximum appeared after a lag period that was 2 3 times longer than that for A23187. In ECV304 clopidogrel at concentrations of 10, 30 and 100 microM produced maximum increment of [Ca2+]i by 16.5 +/- 3.8 nM (n = 7), 47.0 +/- 6.9 nM (n = 8) and 67.2 +/- 8.3 nM (n = 8), respectively. Incubation of BAEC with A23187 (microM), ticlopidine or clopidogrel (100 microM) for 2 h did not influence viability of cultured endothelial cells. We claim that thienopyridines, independently of their delayed anti-platelet properties ex vivo do release NO and PGI2 from cultured endothelial cells in vitro. The above endothelial action of thienopyridines might be mediated by a rise in [Ca2+]i, however, this possibility has not been proved.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/6-Ketoprostaglandin F1 alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Aspirin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Epoprostenol,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrites,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Aggregation Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Ticlopidine,
http://linkedlifedata.com/resource/pubmed/chemical/clopidogrel
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0867-5910
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
50
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
597-604
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10639010-6-Ketoprostaglandin F1 alpha,
pubmed-meshheading:10639010-Animals,
pubmed-meshheading:10639010-Aorta,
pubmed-meshheading:10639010-Aspirin,
pubmed-meshheading:10639010-Calcimycin,
pubmed-meshheading:10639010-Calcium,
pubmed-meshheading:10639010-Cattle,
pubmed-meshheading:10639010-Cell Line,
pubmed-meshheading:10639010-Cell Survival,
pubmed-meshheading:10639010-Cells, Cultured,
pubmed-meshheading:10639010-Dose-Response Relationship, Drug,
pubmed-meshheading:10639010-Drug Interactions,
pubmed-meshheading:10639010-Endothelium, Vascular,
pubmed-meshheading:10639010-Epoprostenol,
pubmed-meshheading:10639010-Humans,
pubmed-meshheading:10639010-Nitrites,
pubmed-meshheading:10639010-Platelet Aggregation Inhibitors,
pubmed-meshheading:10639010-Pyridines,
pubmed-meshheading:10639010-Ticlopidine,
pubmed-meshheading:10639010-Time Factors,
pubmed-meshheading:10639010-Umbilical Veins
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| pubmed:year |
1999
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| pubmed:articleTitle |
Thienopyridines: effects on cultured endothelial cells.
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| pubmed:affiliation |
Jagiellonian University Medical College, Cracow, Poland.
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| pubmed:publicationType |
Journal Article
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