Linked Life Data

10634876

Source:http://linkedlifedata.com/resource/pubmed/id/10634876

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-2-16
pubmed:abstractText
The actions of ethanol (EtOH) on the respiratory output of the neonatal rat brain stem slice preparation in vitro are described. Ethanol inhibited respiratory-related hypoglossal nerve activity in a dose-dependent manner. The effect of EtOH was evident within 5 min and was reversible on EtOH washout. The actions of EtOH were qualitatively similar to those of two other alcohols, methanol and octanol. We investigated the dose-response relationship for each alcohol and determined that the order of potency was methanol < EtOH << octanol, with EC(50) values of 291 mM, 39.7 mM, and 49.2 microM respectively. Application of either strychnine (5 microM) or bicuculline (5 microM) alone, partially but not significantly, reversed the inhibition of respiratory-related hypoglossal nerve activity produced by 50 mM EtOH. Preincubation of rhythmic slices with a combination of both strychnine and bicuculline (both 5 microM) partially, but significantly, blocked the inhibitory actions of EtOH, suggesting that other mechanisms also play a role in the action of EtOH. Preincubation of the slices with 25 microM APV reduced the relative degree of inhibition caused by EtOH suggesting that N-methyl-D-aspartate (NMDA)-receptor-mediated events can be affected by EtOH. Furthermore inhibition of protein kinase C by incubation with 100 nM staurosporine also reduced the efficacy of EtOH. These results suggest that the actions of EtOH may be mediated via glycine, GABA(A), and NMDA receptors and that activation of protein kinase C is involved in the EtOH-induced inhibition of respiratory-related hypoglossal nerve activity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-3077
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
333-42
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10634876-6-Cyano-7-nitroquinoxaline-2,3-dione, pubmed-meshheading:10634876-Animals, pubmed-meshheading:10634876-Animals, Newborn, pubmed-meshheading:10634876-Bicuculline, pubmed-meshheading:10634876-Brain Stem, pubmed-meshheading:10634876-Dizocilpine Maleate, pubmed-meshheading:10634876-Ethanol, pubmed-meshheading:10634876-Hypoglossal Nerve, pubmed-meshheading:10634876-Medulla Oblongata, pubmed-meshheading:10634876-Methanol, pubmed-meshheading:10634876-Protein Kinase C, pubmed-meshheading:10634876-Rats, pubmed-meshheading:10634876-Rats, Sprague-Dawley, pubmed-meshheading:10634876-Receptors, GABA, pubmed-meshheading:10634876-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:10634876-Respiratory Mechanics, pubmed-meshheading:10634876-Staurosporine, pubmed-meshheading:10634876-Strychnine, pubmed-meshheading:10634876-Synaptic Transmission, pubmed-meshheading:10634876-Tetrodotoxin
pubmed:year
2000
pubmed:articleTitle
Effect of ethanol upon respiratory-related hypoglossal nerve output of neonatal rat brain stem slices.
pubmed:affiliation
Department of Physiologycs, School of Medicine, University of Washington, Seattle, Washington 98195-7290, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't