Linked Life Data

10630292

Source:http://linkedlifedata.com/resource/pubmed/id/10630292

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
2000-1-27
pubmed:databankReference
pubmed:abstractText
Members of the gp49-related family of mouse and human immunoglobulin (Ig) superfamily receptors have significant amino acid sequence homology in their C2-type, Ig-like domains and include the killer cell Ig-like receptors (KIRs) for major histocompatibility complex class I molecules. We now report the cloning, complete sequence, and organization of the mouse gp49A gene that encodes the only member of this newly-appreciated family without either of two mutually exclusive functional motifs, namely, immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or a charged transmembrane amino acid for heterodimerization with activation molecules. The gp49A and gp49B genes are 94% identical over 5.6 kilobases, the 5' flanking regions are 94% identical over 1900 nucleotides, and the 3' flanking regions are 97% identical for 121 nucleotides and then diverge completely; the gp49B gene encodes gp49B1 bearing two ITIMs. As measured by flow cytometry with specific antibody, gp49A is expressed on immature bone-marrow-derived mast cells, mature serosal mast cells, and several mouse mast cell lines. The substantial sequence identity of the introns of the gp49A and gp49B genes is comparable to that of the exons, establishing the gene pair as the most homologous of the gp49-related family and suggesting that the gp49A and gp49B genes arose by duplication with relatively little subsequent mutation. The findings also represent the first demonstration that gp49A is expressed on mast cells in tandem with inhibitory gp49B1, and establish that the gp49A gene is not a pseudogene, but rather encodes a protein product with characteristics different from the other family members.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0093-7711
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
286-94
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10630292-Amino Acid Sequence, pubmed-meshheading:10630292-Animals, pubmed-meshheading:10630292-Antigens, Surface, pubmed-meshheading:10630292-Base Sequence, pubmed-meshheading:10630292-Bone Marrow, pubmed-meshheading:10630292-Cell Line, pubmed-meshheading:10630292-Cells, Cultured, pubmed-meshheading:10630292-Conserved Sequence, pubmed-meshheading:10630292-DNA, Complementary, pubmed-meshheading:10630292-Exons, pubmed-meshheading:10630292-Flow Cytometry, pubmed-meshheading:10630292-Humans, pubmed-meshheading:10630292-Immunoglobulins, pubmed-meshheading:10630292-Introns, pubmed-meshheading:10630292-Male, pubmed-meshheading:10630292-Mast Cells, pubmed-meshheading:10630292-Membrane Glycoproteins, pubmed-meshheading:10630292-Mice, pubmed-meshheading:10630292-Mice, Inbred BALB C, pubmed-meshheading:10630292-Models, Genetic, pubmed-meshheading:10630292-Receptors, Immunologic, pubmed-meshheading:10630292-Transfection
pubmed:year
1999
pubmed:articleTitle
The gp49A gene has extensive sequence conservation with the gp49B gene and provides gp49A protein, a unique member of a large family of activating and inhibitory receptors of the immunoglobulin superfamily.
pubmed:affiliation
Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't