Source:http://linkedlifedata.com/resource/pubmed/id/10628402
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2000-1-14
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| pubmed:abstractText |
Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1010-4283
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| pubmed:author |
pubmed-author:AllardW JWJ,
pubmed-author:AnderssonII,
pubmed-author:AndréeRR,
pubmed-author:BørmerO POP,
pubmed-author:BarnettT RTR,
pubmed-author:BeckerCC,
pubmed-author:BelenkyAA,
pubmed-author:BellangerLL,
pubmed-author:DavisGG,
pubmed-author:DowellBB,
pubmed-author:GrauerL SLS,
pubmed-author:HilgersJJ,
pubmed-author:JetteD CDC,
pubmed-author:KarlssonBB,
pubmed-author:KreutzF TFT,
pubmed-author:LaurenLL,
pubmed-author:LeinimaaMM,
pubmed-author:LeinonenJJ,
pubmed-author:LiljaHH,
pubmed-author:LintonH JHJ,
pubmed-author:NagDD,
pubmed-author:PaulBB,
pubmed-author:PellegrinoC MCM,
pubmed-author:StenmanU HUH,
pubmed-author:van der KwastT MTM
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| pubmed:issnType |
Print
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| pubmed:volume |
20 Suppl 1
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
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| pubmed:pagination |
1-12
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10628402-Antibodies, Monoclonal,
pubmed-meshheading:10628402-Cross Reactions,
pubmed-meshheading:10628402-Epitope Mapping,
pubmed-meshheading:10628402-Epitopes,
pubmed-meshheading:10628402-Humans,
pubmed-meshheading:10628402-Immunohistochemistry,
pubmed-meshheading:10628402-Models, Molecular,
pubmed-meshheading:10628402-Prostate-Specific Antigen,
pubmed-meshheading:10628402-Protein Structure, Tertiary,
pubmed-meshheading:10628402-Terminology as Topic
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| pubmed:year |
1999
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| pubmed:articleTitle |
Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen.
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| pubmed:affiliation |
Department of Clinical Chemistry, Helsinki University Central Hospital, Finland.
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| pubmed:publicationType |
Journal Article
|