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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-2-7
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pubmed:abstractText |
The diploid nature of human immunodeficiency virus type 1 (HIV-1) indicates that recombination serves a central function in virus replication and evolution. Previously, while examining the nature of obligatory primer strand transfers during reverse transcription, a high rate of recombination was observed at the ends of the viral genome within the viral long terminal repeats, prompting the following question: does recombination occur at a high rate throughout the genome? To address this question, two vectors based upon different strains of HIV-1 were utilized. The vectors were comprised predominantly of autologous HIV-1 sequence and were approximately the same size as the parental genome. The proviral progeny of heterodimeric virions were analyzed after a single cycle of replication, and the sequence heterogeneity between the two strains allowed direct examination of recombination crossovers. The results obtained indicate that HIV-1 undergoes approximately two to three recombination events per genome per replication cycle. These results imply that both HIV-1 RNAs are typically utilized during reverse transcription and that recombination is an important aspect of HIV-1 replication.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10627533-10329158,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10627533-9986789
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1234-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10627533-Cell Line,
pubmed-meshheading:10627533-DNA, Viral,
pubmed-meshheading:10627533-Genome, Viral,
pubmed-meshheading:10627533-HIV-1,
pubmed-meshheading:10627533-HeLa Cells,
pubmed-meshheading:10627533-Heteroduplex Analysis,
pubmed-meshheading:10627533-Humans,
pubmed-meshheading:10627533-Plasmids,
pubmed-meshheading:10627533-Polymerase Chain Reaction,
pubmed-meshheading:10627533-Proviruses,
pubmed-meshheading:10627533-Recombination, Genetic,
pubmed-meshheading:10627533-Sequence Analysis, DNA,
pubmed-meshheading:10627533-Transfection,
pubmed-meshheading:10627533-Virus Replication
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pubmed:year |
2000
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pubmed:articleTitle |
High rate of recombination throughout the human immunodeficiency virus type 1 genome.
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pubmed:affiliation |
Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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