Linked Life Data

10627304

Source:http://linkedlifedata.com/resource/pubmed/id/10627304

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-3-7
pubmed:abstractText
The present study was undertaken to examine the effect of extracellular pH (pH(0)) on the locomotor function of murine microglial cells in vitro. We have found that basal motility of microglia, as measured by a computer-assisted video assay, decreased in an acidic, but not in an alkaline environment. Extracellular acidification affected the architecture of F-actin cytoskeleton, inducing bundling of actin and the formation of stress fibers. The change in intracellular pH (pH(i)) resulting from the change in pH(0) seems to be a prerequisite for the motility decrease since other means to decrease pH(i), namely Na(+)-free solution (in the absence of HCO(-)(3)) and nigericin-containing solution, mimicked the extracellular acidification. In contrast to its pronounced effect on basal motility of microglial cells, the motility increase, as induced by the chemoattractant complement 5a (C5a), was not affected by the acidic environment. The relationship of pH(0) to the locomotor function was also studied in a long-term microchemotaxis assay where microglia migrated within a pH gradient. Intracellular acidification induced by lowering pH(0) to 6.0 or removal of Na(+) from the assay medium decreased basal microglial cell migration. The C5a-induced chemotactic migration was moderately decreased by the acidic environment. In conclusion, our results suggest that acidification of the microglial extracellular milieu leads to a decrease in pH(i) and thereby reduces the basal microglial motility and C5a-induced chemotaxis via a rearrangement of the cytoskeleton. We would therefore like to speculate that changes in pH(i) constitute an important control mechanism in regulating the locomotor function of microglia in culture and probably also in the intact tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
853
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
22-31
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10627304-Acids, pubmed-meshheading:10627304-Actins, pubmed-meshheading:10627304-Animals, pubmed-meshheading:10627304-Animals, Newborn, pubmed-meshheading:10627304-Antigens, CD, pubmed-meshheading:10627304-Buffers, pubmed-meshheading:10627304-Cell Movement, pubmed-meshheading:10627304-Cell Survival, pubmed-meshheading:10627304-Cells, Cultured, pubmed-meshheading:10627304-Chemotaxis, pubmed-meshheading:10627304-Complement C5a, pubmed-meshheading:10627304-Cytochalasin B, pubmed-meshheading:10627304-Cytoskeleton, pubmed-meshheading:10627304-Extracellular Space, pubmed-meshheading:10627304-Fluoresceins, pubmed-meshheading:10627304-Fluorescent Dyes, pubmed-meshheading:10627304-Hydrogen-Ion Concentration, pubmed-meshheading:10627304-Intracellular Fluid, pubmed-meshheading:10627304-Mice, pubmed-meshheading:10627304-Mice, Inbred Strains, pubmed-meshheading:10627304-Microglia, pubmed-meshheading:10627304-Microscopy, Video, pubmed-meshheading:10627304-Receptor, Anaphylatoxin C5a, pubmed-meshheading:10627304-Receptors, Complement
pubmed:year
2000
pubmed:articleTitle
Extracellular acidification decreases the basal motility of cultured mouse microglia via the rearrangement of the actin cytoskeleton.
pubmed:affiliation
Medical Research Center, Polish Academy of Sciences, 5 Pawinskiego Str., 02-106, Warsaw, Poland. lidia.faff@sci.monash.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't