Linked Life Data

10626218

Source:http://linkedlifedata.com/resource/pubmed/id/10626218

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
150
pubmed:dateCreated
2000-2-17
pubmed:abstractText
The exocyclic cytosine adduct 3,N4-ethenocytosine is highly mutagenic in mammalian cells. We describe the impact of this adduct on DNA duplex stability. The adduct does not disrupt the overall B-form DNA structure; however, structural accommodation of the adduct is necessary at the lesion site. Despite the relatively small structural perturbation imparted by the adduct, there is a large adduct-induced destabilization of the DNA duplex. This destabilization is observed to be independent of the cross-strand partner base and neighbouring base pairs. The thermodynamic origins of the destabilization are, however, strongly dependent on the cross-strand partner base and neighbouring base pairs. Comparisons are made between the impact of the 3,N4-ethenocytosine adduct and other lesions on DNA thermodynamics. The lesions are similar in that all result in destabilization of the DNA duplex. The magnitudes and the thermodynamic origins of that destabilization vary widely, the 3,N4-ethenocytosine adduct being dramatically more destabilizing than other lesions. The impact of damaged sites on the stability of the DNA helix suggests that energetic differences between damaged and normal DNA may contribute to the recognition of damage by the cellular DNA repair machinery.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-5038
pubmed:author
pubmed:issnType
Print
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-77
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Effects of 3,N4-ethenodeoxycytidine on duplex stability and energetics.
pubmed:affiliation
Department of Chemistry, Rutgers, State University of New Jersey, Piscataway 08854-8087, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review